目的研究不同扩繁模式对朝鲜淫羊藿有效成分累积的影响,为朝鲜淫羊藿规范化栽培提供理论依据。方法采用紫外可见分光光度法与HPLC法测定不同扩繁模式下朝鲜淫羊藿中总黄酮和淫羊藿苷的量。结果不同扩繁模式下,朝鲜淫羊藿有效成分量存在显著差异,50%遮光人工栽培和自然光下人工栽培的总黄酮和淫羊藿苷量受采收期和采收年限的影响、较小,有效成分量高。结论朝鲜淫羊藿以50%遮光人工栽培扩繁模式最佳。
Objective To study the effects of the different propagation modes on the accumulation of active components in Epimedium koreanum, and to provide the guidance for the standardized cultivation ofE. koreanum. Methods The contents of total flavonoids and icariin in the different propagation modes orE. koreanum were measured by HPLC and ultraviolet spectrophotometry. Results The contents of the active components had the significant difference in E. koreanum of different propagation modes. By cultivating under the 50% man-made shade and under the sun, the contents of the active components were far higher and more stable, but little impacted by harvest time and growth year. Conclusion When cultivated under the 50% man-made shade, E. koreanum is the best of the four propagation modes.