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中文摘要：
目的：研究流感病毒在体外对神经元的感染及活性影响,检测流感病毒在神经元内的复制效率。方法：原代培养小鼠神经元细胞,β-tubulin III抗体进行免疫荧光鉴定小鼠神经元纯度,流感病毒吸附、培养;荧光定量PCR检测神经元内病毒载量;CCK-8检测流感病毒对神经元活性的影响。结果：免疫荧光结果表明,神经元纯度大于95%。CCK-8检测结果显示,流感病毒对神经元感染可显著抑制神经元细胞活性。荧光定量PCR结果表明,流感病毒可感染神经元并在其细胞内进行病毒复制。结论：流感病毒可在体外感染小鼠原代神经元进行病毒复制,并影响神经元活性。
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