中文摘要：

目的观察益气活血对脑出血大鼠脑内损伤区血管内皮生长因子（VEGF）mRNA及其受体Flt—1 mRNA和Flk—1 mRNA表达的影响，探讨益气活血法的作用机制。方法通过立体定位向脑内苍白球注入Ⅶ型胶原酶0．5U建立脑出血模型。SD大鼠随机分为正常组、假手术组、模型组、益气活血组、益气组、活血组，分别灌服益气活血法代表方补阳还五汤全方及其益气组分、活血组分药物（剂量为临床70kg成人用量的3倍），运用逆转录聚合酶链式反应（RT—PCR）检测正常组及其它各组于造模术后1、4、7、14、21、28d各时间点VEGFmRNA、Flt—1 mRNA和Flk—1 mRNA的动态变化。结果正常组及假手术组不同时间点VEGFmR．NA、Flt—1 mRNA和Flk—1 mRNA均未见明显表达；模型组VEGFmRNA、Flt-1 mRNA和Flk—1 mRNA在脑出血后1d即开始表达，21d达到高峰，然后逐渐减弱；益气活血组VEGFmRNA、Flt—1 mRNA和Flk—1 mRNA表达在7～21d明显高于其他各组，VEGFmRNA和Flk—1 mRNA表达在术后14d达高峰，Flt—1 mRNA在术后21d达高峰。结论益气活血法能促进脑出血大鼠脑内VEGFmRNA、Flt—1 mRNA和Flk—1 mRNA的表达而调整血管新生过程，可能是其促进组织修复的机制之一。

英文摘要：

Objective： To investigate the mechanisms of Yi - Qi Huo - Xue therapy（YQHX） by observing its effects on expressions of the mRNA of vascular endothelial growth factor （VEGF）and the receptors Flt - 1, Flk - 1 in intracerebral hemorrhagic（ICH） rat brains. Methods： ICH was induced by injecting collagenase type Ⅶ stereotaxically into rat brain right globus pallidus. One hundred and fifty- five Sprague-Dawley rats were randomly divided into six groups, including normal control group（ n = 5）, sham group（ n = 30）, ICH model group（ n = 30）, YQHX - treated group（ n = 30）, YiQi - treated group（ n = 30） and HuoXue - treated group（ n = 30）, respectively administered orally with the classic YQHX prescription, Buyanghuanwu decoction, its YiQi and Huo xue compo- nents, all at the dosage three times that of a 70kg adult; The expression of mRNA of VEGF, FLT - 1 and Flk - 1 was assayed by reverse transcription- polymerase chain reaction（RT- PCR）at day 1,4,7,14,21 and 28 after the onset. Results： Nonsignifficant expression of VEGF mRNA in rat brains of normal or sham during the experiment; In model group, the expressions of VEGF mRNA,Flt - 1mRNA and Flk - 1mRNA appeared at ld, peaked at 21d, and then weakened gradually; The expressions of VEGF mRNA,Flt- 1mRNA and Flk - 1mRNA in YQHX -treated group were significantly higher than those in the other groups from 7d to 21d, and the expression of VEGF mRNA and Flk - 1mRNA in YQHX-treated group reached peak at 14d, but the expression of Flt - 1mRNA reached at 21 d. Conclusions： The Yi - Qi Huo - Xue therapy can promote the expressions of VEGFmRNA ,Flt - 1 mRNA and Flk -1mRNA in intracerebral hemorrhagic rats, which may be related with its promoting the reconstruction of the microvascular networks in the damaged zone.