中文摘要：

用热水煮提法提取出柚皮中柚皮苷和类柠檬苦素等大量苦味活性物质,通过静态吸附-解吸试验,筛选出具有较大吸附和解吸率的D101大孔吸附树脂进行分离纯化,得出最优的吸附条件：树脂添加量3.0%（质量分数）,25℃恒温条件下摇床以180 r/min振荡90 min.在此条件下,柚皮苷和柠檬苦素吸附率都达到100%.吸附完全后进行动态洗脱,洗脱液再通过萃取和重结晶后可有效分离纯化柚皮苷和柠檬苦素类似物,纯化后的样品用HPLC法测得柚皮苷纯度达到95.8%,用紫外分光光度法测得柠檬苦素类似物纯度达到90.9%.

英文摘要：

Bitterness active components such as naringin and limonoids were extracted by heating water.By means of adsorption-desorption experiments,the macro porous resin D101 was selected for isolation and purification of naringin and limonoids from pomelo peel due to its higher adsorption and desorption rate. The adsorption condition was optimized in the presence of 3% resin with absorption time of 90 min,at 25 ℃ and rotation speed of 180 r / min. After complete adsorption,the resulting solution was applied to dynamic-state elution. Naringin and limonoids were separated and purified effectively via concentration of the eluent and recrystalization. The samples thus prepared were determined by HPLC and UV spectrophotometer,the purities of naringin and limonoids reached 95. 8% and 90. 9%,respectively.