中文摘要：

该文研究了白藜芦醇及其下游信号分子沉默信息调节因子1（silentinformationregulator1，SIRTI）对高糖培养条件下人肾小管上皮细胞（HK-2）转化的作用和机制。体外常规培养HK．2细胞，采用Westernblot检测平滑肌肌动蛋白（α-SMA）、E-钙黏着蛋白（E-cadherin）TL信号蛋白SIRT1、过氧化物酶体增殖物激活受体v协同刺激因子一1a（peroxisomeproliferator-activatedreceptorgammacoactivator-1α，PGC—lu）的蛋白表达，采用细胞免疫荧光对SIRT1的表达进行检测。与高糖刺激0h组相比，高糖刺激12，24，48h均导致HK-2细胞SIRT1蛋白明显减少，且随时间呈下降趋势：低糖培养细胞0，12，24，48h的SIRT1蛋白表达没有明显差异。白藜芦醇明显提高高糖培养条件下HK-2细胞的SIRT1表达，而SIRT1特异性抑制剂EX527能够减弱白藜芦醇的作用。进一步的研究表明，白藜芦醇能够明显增加HK-2细胞中E-钙黏着蛋白的表达，抑制高糖导致的α-SMA表达升高，而EX527对高糖诱导的HK-2细胞转分化没有显著影响。此外，研究发现，白藜芦醇能够明显增加高糖刺激下HK-2细胞PGC-1α蛋白表达。该研究结果提示，白藜芦醇可能通过SIRTl和PGC—1α信号通路抑制了高糖诱导的HK-2细胞转化过程。

英文摘要：

We investigated the role of resveratrol and SIRT1 signaling pathway on epithelial to mesenchymal transition of human renal proximal tubular epithelial cells （HK-2） cultured with high glucose. HK-2 cells were cultured in vitro. The protein levels of α-SMA, E-cadherin, SIRT1 and PGC-1αwere determined by Western blot. The expression level of SIRT1 was also examined by immunofluorescence assay. Compared with 0 h, the exposure of HK-2 cells to high concentration of glucose resulted in decreased protein levels of SIRT 1 in a time-dependent manner. But HK-2 cells incubated with low concentrations of glucose for various periods didn＇t show a time-dependent decrease in SIRT1 expression. Resveratrol treatment resulted in increased protein level of SIRT1. This effect was markedly diminished by the addition of EX527, an inhibitor of SIRT1. Furthermore, resveratrol treatment also resulted in increased protein level of E-cadherin, along with a down-regulation of α-SMA, although EX527 alone had no effect on high glucose-induced EMT of HK-2 cells. In addition, resveratrol treatment also prevented the decrease of PGC-1 α in HK-2 cells cultured with high glucose. Taken together, these data demonstrated that resveratrol could prevent high glucose-induced EMT of human renal proximal tubular epithelial cells via activation of SIRT1 and PGC- 1 α signaling pathway.