中文摘要：

引物设计是PCR技术中最重要的一环。本文就引物设计前的准备工作、引物设计原则、引物设计软件和方法等相关问题，介绍在动物传染病研究中设计PCR引物的一些应用体会。

英文摘要：

PCR-primer design is the most important step in polymerase chain reaction （PCR） technique. Preparation beforehand, the principal and method of PCR-primer design, the usage skill of popular primer-design software and some correlative matter in the study of animal infectious disease were introduced detailedly.