为了验证白腐真菌对杀菌剂——百菌清的降解能力,并初步探讨其降解机制,以平皿培养方式对70株白腐真菌菌株降解百菌清的能力进行验证和筛选,并选择降解作用突出的一色齿毛菌Cerrenaunicolor Y4941进行液体培养条件下对百菌清的降解。结果表明:卵菌杀菌剂没有对测试的白腐真菌产生抑制或杀灭作用,在所筛选的70株菌株中,一色齿毛菌Cerrena unicolor Y4941、硬毛粗盖孔菌Funaliatrogii W4289、桦褶孔菌Lenzitesbetulina Y4962、藏红硬孔菌RigidoporuscrocatusD10146、裂褶菌Schizophyllum commune D9466和云芝栓孔菌Trametes versicolor W4614等菌株都对百菌清显示出较强的降解能力;对一色齿毛菌Cerrena unicolor Y4941在液体培养条件下降解百菌清研究的实验结果表明,活菌(1ivin8culture,LC)降解率〉粗酶液(crudeenzyme liquor,CEL)降解率〉灭活菌丝体(devitalizedmycelia,DM)降解率,经过活菌降解3d的样品中百菌清的残留率仅为1.92%;对培养液中漆酶活性测定结果显示,漆酶活性最高可达2195.55U/L。上述证明,杀菌剂百菌清不对白腐真菌产生抑制或杀灭作用,相反,一色齿毛菌Cerrena unicolor Y4941等菌株对其有很强的降解能力,在杀菌剂生物降解领域有潜在的应用前景。
The degradation ability of white-rot fungi to chlorothalonil and the degradation mechanism of chlorothalonil were nvestigated. By using agar plate culture method, the degradation capacities to chlorothalonil of 70 strains of white-rot fungi were verified, of which Cerrena unicolor Y4941 was studied emphatically using shake and liquid culture. Laccase activity assay was also employed in this study. The result proved that the tested white-rot fungi were not inhibited or eliminated by chlorothalonil. Among these strains, Trametes versicolor W4614, Funafia trogii W4289, Lenzites betulina Y4962, Rigidoporus crocatus D10146, Schizophyllum commune D9466 and Cerrena unicolor Y4941 showed strong capacities to degrade chlorothalonil. The degradationcapability of strain Cerrena unicolor Y4941 decrease in order of living culture (LC) 〉crude enzyme liquor (CEL) 〉devitalized mycelia (DM). The lowest chlorothalonil residue of 1.92% was detected in 3 days of degradation. Enzyme assay showed the laccase activity reached 2 195.55U/L in liquid culture media. Cerrena unicolor Y4941 showed the highest degradation capability. The result suggests that white-rot fungi have potential application value in biodegradation of fungicides