中文摘要：

目的合成一种2-脱氧-D-葡萄糖（2-DG）包被γ-Fe2O3纳米探针,检测其性质特征及对前列腺癌PC3细胞葡萄糖受体的靶向性摄取。方法运用化学共沉淀法合成γ-Fe2O3@二巯基丁二酸（DMSA）,再与2-DG共轭生成γ-Fe2O3@DMSA-DG,红外光谱和透射电镜检测粒子结构特征;噻唑蓝试验（MTT）法进行细胞毒性实验,分别加入含无糖DMEM的探针培养基γ-Fe2O3@DMSA、γ-Fe2O3@DMSA-DG、γ-Fe2O3@DMSA-DG＋2-DG（4.5 mg/mL）,将探针在不同时间（10 min、20 min、1 h、2 h）孵育PC3细胞,运用普鲁士兰染色法和铁三嗪法分析PC3细胞对探针的吸收情况,并以体外MRI观察T2WI信号强度变化。结果 2-DG以酰胺键连接γ-Fe2O3@DMSA,粒子平均直径10 mm;探针未见明显毒性;γ-Fe2O3@DMSA-DG可被PC3细胞靶向摄取,并可在早期被2-DG抑制。结论本研究成功合成γ-Fe2O3@DMSA-DG探针,其对PC3细胞葡萄糖受体有较好的靶向性,使用临床型MR仪可对其进行监测。

英文摘要：

Objective To synthetize novel glut-targeted γ-Fe2O3 NPs coated with DMSA,in which modied with 2-DG,then to evaluate its characteristics and the prostatic cancer PC3 cells glut-targeting uptake of the γ-Fe2O3@DMSA-DG.Methods The γ-Fe2O3@DMSA was synthesized by chemical precipitation,then modified with 2-DG to prepare γ-Fe2O3@DMSA-DG.The nanoparticles were observed using IR spectra and transmission electron microscope.The nanoparticles cytotoxicity was investigated using the 3-（4,5-Dimethy-lthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay.PC3 cells were incubated with γ-Fe2O3@DMSA-DG NPs,γ-Fe2O3@DMSA NPs or γ-Fe2O3@DMSA-DG NPs in the presence of D-glucose for different time points（10 min,20 min and 1 h,2 h）.The uptake efficiency of intracellular iron was measured by Prussian blue staining and the ferrozine assay.The T2WI signal of PC3 cells after incubating was monitored by 1.5 T systems MR scanner.Results The 2-DG was connected with γ-Fe2O3@DMSA by amide linkage and its diameter was 10 nm;the nanoparticles showed low toxicity,γ-Fe2O3@DMSA-DG was uptaken targetingly by PC3 cells,and which was inhibited by the glucose receptor at early time points.Conclusion This study synthetize γ-Fe2O3@DMSA-DG NPs successfully,which has good targeting ability to the glut-expressing of PC3 cells,and the cell targeting events of the probe can be monitored by using a clinical MR scanner.