中文摘要：

通过PCR方法扩增出HCVNS3—5b全长基因序列，克隆入真核表达载体pIRES2-EGFP中，构建重组质粒p1RES2-EG，FP—NS3—5b。利用脂质体将该质粒转染至BHK-21细胞，通过荧光成像和WesternBlot检测NS3—5b基因的表达。结果显示成功构建了真核表达载体pIRES2-EGFP—NS3—5b，并且NS3／4A蛋白和NS5B蛋白得到特异性表达，为下一步建立HCVRdRp活性的细胞评价系统和动物模型评价系统奠定了实验和理论基础。

英文摘要：

To construct the recombinant plasmid pIRES2-EGFP-NS3-SB, HCV NS3-Sb gene was amplified by the polymerase chain reaction （PCR） and cloned into the eukaryotic expression plasmid plRES2-EGFP. Then the recombinant plasmid was transfected into BHK-21 cells by Lipofectamine 2000 and the expression of target genes were identified by fluorescence imaging and western blot analysis. The results showed that the plasmid pIRES2-EG- FP-NS3-5b was successfully constructed and NS3/4A and NSSB proteins were specifically expressed in the eukary- otic cells. It provides the basis for the establishment of a cell-based HCV RdRp evaluation system and animal mod- els development.