中文摘要：

参照文献方法合成了BSA修饰的水溶性发光金纳米粒子,并考察了其与溶菌酶之间的相互作用。依据溶菌酶对金纳米粒子的发光增强现象,建立了测定溶菌酶的荧光新方法。考察了发光金纳米粒子的浓度、pH值、反应时间及共存物质对测定的影响。优化条件为：发光金纳米粒子浓度4.0×10^-6 mol/L、pH 7.0、反应时间10 min。在此条件下,荧光增强与溶菌酶浓度在2×10^-7～8×10^-6 mol/L范围内呈良好的线性关系,检出限为3.0×10^-8 mol/L。对4.0×10^-6 mol/L溶菌酶平行测定6次,相对标准偏差为2.6%。本方法具有灵敏度高、探针水溶性好和生物毒性低的优点,同时,常见低等电点蛋白质对分析不干扰。本方法用于合成样品及鸡蛋清中溶菌酶含量测定,平均回收率为97.5%～103.6%。

英文摘要：

As lysozyme has the property of dissolving some bacteria,it can be applied in medical treatment,biological engineering,and especially in food antisepsis to replace chemically synthesized ones.Therefore,the development of a simple analytical method for lysozyme assay is very important.Here,water-soluble BSA-modified fluorescent gold nanoparticles（GNPs） were prepared according to the literature,and their interaction with lysozyme was investigated.A novel fluorescence method for the determination of lysozyme has been developed based on the enhancement effect of lysozyme on the fluorescence of GNPs.The effects of GNPs concentration,pH,reaction time and coexisting substances on the determination were tested.Under the optimum conditions,i.e.4.0 ×10^-6 mol/L GNPs,pH 7.0,and reaction time of 10 min,the linear range of the calibration curve was from 2×10^-7 mol/L to 8×10^-6 mol/L and the detection limit was 3.0×10^-8 mol /L.The relative standard deviation was 2.6% for six replicate measurements of a solution containing 4.0×10^-6 mol/L lysozyme.The method bore the merits of high sensitivity,good water-solubility and low-toxicity of probe.Meanwhile,some common proteins with low isoelectric point almost did not interfere with the determination.The proposed method was applied to the determination of lysozyme in synthetic samples and chicken eggs white,the average recoveries were 97.5%-103.6% were obtained.