中文摘要：

<正>The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on Th1-Th2 balance modulation.However,it is unclear whether or not vitamin A can regulate Th1-Th2 balance under a strong Th1-polarizing condition.Therefore,the purpose of our study was to examine the effect of vitamin A metabolite all-trans retinoic acid(ATRA)on Th1-Th2 differentiation in CD4+T cells under GATA-3 deficiency,which can induce Th1-polarizing condition.In the present study,GATA-3 deficiency T cells were induced by siRNA and checked by

英文摘要：

The essential effect of vitamin A on immune function occurs through various mechanisms including direct effect on ThloTh2 balance modulation. However, it is unclear whether or not vitamin A can regulate Thl-Th2 balance under a strong Thl-polarizing condition. Therefore, the purpose of our study was to examine the effect of vitamin A metabolite allotrans retinoic acid （ATRA） on ThloTh2 differentiation in CD4~ T cells under GATA-3 deficiency, which can induce Thl-polarizing condition. In the present study, GATA-3 deficiency T cells were induced by siRNA and checked by real-time quantitative PCR and western blot. GATA-3 deficiency CD4＋ T cells and normal CD4＋ T were treated for 48 h with or without ATRA.