中文摘要：

由于大豆胞囊线虫（soybean cyst nematode，SCN）特殊的生长环境，用细菌专用DNA试剂盒提取方法提取DNA的质量不理想，而且耗时长，本文介绍了一种快速提取线虫DNA的方法。通过比较细菌专用DNA试剂盒提取法、质粒试剂盒提取法和CTAB法，发现利用质粒提取试剂盒，改良提取步骤，可以快速的提取质量较好的线虫DNA，rDNA-ITS区域序列扩增可有效扩增出目的基因，该方法可以直接用于线虫基因获取试验。

英文摘要：

In this paper, we reported a method for rapid extraction of DNA from soybean cyst nematode（SCN）. It is difficult and time consuming to obtain high quality DNA by using bacterial genomic DNA extraction kit for the special growth environ- ment of SCN. Three methods for extraction of DNA were compared in this study, including Bacterial genomic DNA extraction kit, San Prep column plasmid DNA Kit and CTAB method. Results showed that DNA isolated by improved San Prep column plasmid DNA Kit was high quality with time saving. We verified the quality by amplification sequence effectively from rDNA- ITS region. The method could be used for DNA obtaining from cyst and the later molecular manipulations of genes cloning.