中文摘要：

研究携带TRAIL和Smac的双基因溶瘤腺病毒（ZD55-TRAIL-IETD-Smac）联合化疗药物5-氟尿嘧啶（5-FU）对肺癌细胞的体外杀伤作用。通过MTT法检测细胞抑制率,流式细胞术检测细胞凋亡率,Western Blot检测杀伤性基因表达与凋亡相关蛋白表达。结果显示ZD55-TRAIL-IETD-Smac与5-FU联合应用能有效地抑制肺癌细胞的增殖,并通过激活Caspase通路诱导肺癌细胞产生凋亡,表明,5-FU能够显著增强携带TRAIL和Smac的双基因溶瘤腺病毒对肺癌细胞的杀伤作用,为肺癌的临床应用提供了参考。

英文摘要：

The study explores the in-vitro lethal effects of dual-gene oncolytic adenovirus（ZD55-TRAIL-IETD-Smac）with TRAIL and Smac in combination of 5-FU on lung cancer cells.Cell inhibition ratio,apoptosis rate,destruction gene expression apoptosis-related protein expression were detected by MTT,flow cytometry and Western Blot respectively.The results show that combined application of ZD55-TRAIL-IETD-Smac and 5-FU can effectively inhibit the proliferation of lung cancer cells and induce apoptosis of lung cancer cells through activating Caspase passage.This indicates that 5-FU can significantly enhance lethal effects of dual-gene oncolytic adenovirus with TRAIL and Smac on lung cancer cells.This provides reference for clinical application of lung cancer.