中文摘要：

人的免疫不全 virus-1 (HIV-1 ) 编码 15 病毒的蛋白质。蛋白质蛋白质相互作用在这些蛋白质的功能起一个大作用。在这研究，我们试图识别在 HIV-1 蛋白质之间的新奇相互作用更好理解角色在 HIV-1 的生命周期由病毒的蛋白质蛋白质相互作用玩了。从 HIV-1 紧张 AD8 编码 15 病毒的蛋白质的基因被插入到酵母的 plasmids 二混血儿的系统。由屏蔽 120 蛋白质，在七对之间的相互作用被发现。这导致了在 HIV-1 蛋白质 integrase 之间的一个相互作用的发现(在里面) 并且 glycoprotein 41 (gp41 ) ，它被试金和荧光回声精力转移的两 co-immunoprecipitation (Co-IP ) 证实(烦恼) 在实时房间的成像。另外，在 gp41 的位置 76-100 的氨基酸被要求让它绑在，这被发现在里面。从 gp41 的这个区域的删除阻止了它的相互作用与在里面并且在 293T 房间减少了 HIV-1 的生产。这研究在改进 gp41 并且在的生物功能的理解的 HIV-1 蛋白质蛋白质相互作用上提供新信息在病毒生命周期期间。

英文摘要：

Human immunodeficiency virus-1 （HIV-1）encodes 15 viral proteins. Protein-protein interactions play a large role in the function of these proteins. In this study, we attempted to identify novel interactions between the HIV-1 proteins to better understand the role played by viral protein-protein interactions in the life cycle of HIV-I. Genes encoding the 15 viral proteins from the HIV-1 strain AD8 were inserted into the plasmids of a yeast two-hybrid system. By screening 120 pairs of proteins, interactions between seven pairs were found. This led to the discovery of an interaction between the HIV-1 proteins integrase （IN） and glycoprotein 41 （gp41）, which was confirmed by both co-immunoprecipitation （Co-IP） assays and fluorescence resonance energy transfer （FRET） imaging in live cells. In addition, it was found that the amino acids at positions 76-100 of gp41 are required for it to bind to IN. Deletion of this region from gp41 prevented its interaction with IN and reduced the production of HIV-1 in 293T cells. This study provides new information on HIV-1 protein-protein interactions which improves the understanding of the biological functions of gp41 and IN during the virus life cycle.