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中文摘要：
在pH7.4的缓冲溶液中,采用紫外-可见、荧光光谱法和DNA熔点实验并结合荧光探针亚甲基蓝（MB）,研究杜鹃素与小牛胸腺DNA的结合模式以及Cu^2＋、Co^2＋和Mg^2＋分别对结合模式的影响。结果表明：杜鹃素主要以嵌插模式与DNA结合。3种金属离子均能不同程度影响杜鹃素的吸收光谱和增强DNA-MB复合物的荧光强度。随着体系中Cu^2＋浓度的增加,DNA-杜鹃素复合物的结合常数呈现出先减小后增加的趋势,增加Mg^2＋的用量则使复合物的结合常数影响先增强然后缓慢减弱,而Co^2＋浓度的增大对复合物的结合常数影响较小。由此得出,Cu^2＋、Co^2＋和Mg^2＋对该DNA-杜鹃素结合的影响分别取决于金属离子与杜鹃素的结合能力以及与DNA的碱基和磷酸基团间的结合程度。
英文摘要：
The interaction between farrerol and calf thymus DNA（ctDNA） was explored by UV-visible spectrophotometry,fluorescence spectroscopy,and melting-point technique in combination with methylene blue（MB） competition in the presence of Cu^2＋,Co^2＋ and Mg^2＋ in physiological buffer（pH 7.4）.The results revealed that farrerol could be intercalatively bound to DNA.Cu^2＋,Co^2＋ and Mg^2＋ had different effects on the absorption spectrum of farrerol and could increase the fluorescence intensity of DNA-MB complex.A gradual increase in Cu^2＋ concentration could result in an initial decrease followed by an increase in the binding constant of DNA-farrerol complex.In the presence of higher Mg^2＋ concentration,the binding constant of DNA-farrerol complex tended to initially increase and then decrease.However,Increasing Co^2＋ concentration revealed little effect on the binding constant of DNA-farrerol complex.Therefore,the effect of metal ions on the binding between farrerol and DNA mainly depends on the relative affinity between metal ions and farrerol,DNA basic groups or phosphate groups.
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