发展建立新型的高通量分析方法是生命科学研究中海量组分检测的关键。表面等离子体共振成像(SPRI)作为新发展起来的高灵敏分析技术,能以微阵列形式实时检测研究各种分子作用的过程,是一种非常有潜力的多功能高通量研究平台。本文对SPRI的基本原理及其在膜性能和生物分子相互作用研究中的应用作了较详细的评述,重点讨论了SPRI的最新发展情况,并对其发展前景进行了展望。
This review introduces a novel microdot sensing method,surface plasmon resonance imaging(SPRI),which is a label-free method suitable for in situ and real time monitoring of molecular interaction events simultaneously.It is becoming a new and promising platform for high-throughput study of a huge amount of biological samples with limited volume or resources.In addition to the introduction of its working principle,method development,and applications to the characterization of thin film and biomolecular interaction monitoring,the state-of-art of this method is particularly focused and discussed to have an insight into its future development trends.