中文摘要：

Ryanodine受体1（RyR1）在骨骼肌细胞的兴奋．收缩偶联过程中扮演重要角色，是肌质网快速释放Ca^2＋的通道．RyR1的结构研究需要大量的且纯度很高的蛋白．目前，文献中的方法不能一次性得到大量的纯化蛋白，因而不能满足结构研究的需要．通过运用肝素（heparin，HP）层析与羟基磷灰石（hydroxyapatite，HA）层析，可以在1d内纯化获得毫克级RyR1．SDS-PAGE显示，RyR1纯度达95％以上．在透射电子显微镜下观察到RyR1是一个正方形的结构，形态类似儿童玩具风车．这些结果表明，该纯化方法获得的RyR1不仅纯度高而且结构完整．

英文摘要：

Ryanodine receptor 1 （RyR1） plays a vital role in excitation-contraction coupling in skeletal muscle. It is the fast Ca^2＋ release channel of the sarcoplasmic reticulum. Large amount of purified protein is required for structural studies of RyR1. Currently, the methods in literature are all small scale and cannot fulfill the requirement for structural studies. By using fast flow heparin （HP）and hydroxyapatite （HA） chromatography, we purified the RyR1 in milligram scale in one day. SDS-PAGE showed that the purity is over 95 % ; electron microscopy revealed that the purified RyR1 molecule displays a square-like structure with an appearance of a pinwheel. These data demonstrate that both purify and structural integrity is achieved by this fast method.