中文摘要：

目的建立鄂产竹节参药材的高效液相色谱法指纹图谱。方法采用Apollo C18（4．6mm×250mm，5μm）色谱柱，乙腈-0．05％磷酸水溶液为流动相，梯度洗脱，流速1mL·min^-1，检测波长203nm，柱温：30℃。结果测试样品10批，以竹节参皂苷V色谱峰为参照峰，确立4个共有峰作为竹节参药材特征峰，测得其含量分别为12．616，3．976，5．625，4．429mg·g^-1。结论竹节参药材特征图谱专属性强，可用于鄂产竹节参药材的鉴定及质量控制。

英文摘要：

Objective To establish the HPLC finger-print for Panax japonicus produced in Hubei. Methods The analysis was performed on a Apollo Cls column（4.6 mm×250 mm,5 μm） ,using the mobile phase of acetonitrile-0.05% solution phosphoric acid; the detection wavelength was 203 nm; the column temperature was 30 ℃ and the flow rate was 1 mL . min^-1. Results Ten samples collected in different picking time common peaks were confirmed in specific chromatograms. Four compounds were elucidated with the peak of Chikusetsu saponin V as reference. The content of 4 major common peaks were 12. 616,3. 976,5. 625, and 4. 429 mg . g^-1, respectively. Conclusion The method can be applied to the quality control of Panax Japonicum C. A. Mey. in Hubei.