中文摘要：

[目的]探讨Pyrin重组蛋白对博来霉素诱导的小鼠肺纤维化NF-κB信号通路的作用.[方法]选择雌性清洁级BABL/c小鼠,随机分为正常对照组、肺纤维化模型组及Pyrin重组蛋白治疗组.第14 d给小鼠施行全身麻醉取右肺组织行HE及Masson三色染色.用Western blot检测肺组织中NF-κB p65蛋白的表达.[结果]与正常对照组比较,肺纤维化模型组小鼠肺组织中NF-κB p65蛋白表达水平显著升高（P〈0.01）;与肺纤维化模型组比较,Pyrin重组蛋白治疗组肺组织中NF-κB p65蛋白水平显著降低（P〈0.05）.[结论]Pyrin重组蛋白可抑制肺纤维化的发生,其机制可能是通过阻断NF-κB信号通路实现的.

英文摘要：

OBJECTIVE To explore the intervention effect of Pyrin recombinant protein on pulmonary fibrosis of mice induced by bleomycin.METHODS30 female BABL/c mouse of clean grade were randomly divided into three groups,10 mouse in each group,respectively,the saline control group,pulmonary fibrosis model group and Pyrin recombinant protein therapy for 14 days group.Take the left lung tissue of mice to stained by Hematoxylin eosin（HE）and PAS under the general anesthesia on fourteenth day.The expression of NF-κB protein of lung tissue were tested by Western blot.RESULTS Pulmonary fibrosis model group compared with control group,the expression of NF-κB protein were significantly higher（P〈0.01）.Pyrin recombinant protein therapy for 14 days group compared with pulmonary fibrosis model group,the expression of NF-κB protein were decreased（P 0.05）.CONCLUSION Pyrin recombinant protein may inhibit pulmonary fibrosis and its mechanism may block the signal transduction pathway of NF-κB.