中文摘要：

在延边黄牛体细胞克隆胚胎体外培养液中分别添加H2O2和GSH,观察重组胚体外发育情况,探究二者对延边黄牛体细胞克隆胚胎氧化损伤的影响。结果表明：不同时间添加H2O2时0～24 h组卵裂率显著高于其它组（P〈0.05）,48～72 h组16细胞以上发育率显著低于其它处理组（P〈0.05）。不同时间不添加H2O2时48～72 h组16细胞期以上发育率显著高于0～24 h组和24～48 h组（P〈0.05）,24～48 h组显著高于0～24 h组（P〈0.05）。不同时间添加GSH时0～24 h组和24～48 h组卵裂率显著高于对照组、48～72 h组和72～96 h组（P〈0.05）,对照组16细胞以上发育率显著低于48～72 h组和72～96 h组（P〈0.05）。结论：H2O2对延边黄牛体细胞克隆胚胎发育前期的氧化损伤较大,特别是在48～72 h。培养前期添加1 mmol/L的GSH能够有效提高延边黄牛体细胞克隆胚胎体外发育效率,缓解有害应激带来的氧化损伤。

英文摘要：

After adding H2O2 and GSH into cultural medium in vitro of somatic cell cloning embryos,the oxidative damage of somatic cell cloning embryos of Yanbian cattle was studied.The results showed that when adding H2O2 in different time,the cleavage rate of the 0-24 h group was significantly higher than that of the other groups（P0.05）,the developmental rate of over 16-cell of the 48-72 h group was significantly lower than that of the other groups（P0.05）.Without H2O2 in different time,the developmental rate of over 16-cell of the 48-72 h group was significantly higher than that of the group of 0-24 h and 24-48 h（P0.05）,that of the 24-48 h group was significantly higher than that of the 0-24 h group（P0.05）.With GSH in different time,the cleavage rates of the 0-24 h group and the 24-48 h group were significantly higher than that of the control group,the 48-72 h group and the 72-96 h group（P0.05）,respectively.The developmental rates of over 16-cell of the control group was significantly lower than that of the 48-72 h group and the 72-96 h group（P0.05）.In conclusion：oxidative damage of H2O2 to somatic cell cloning early embryos of Yanbian cattle was serious,especially in the period of 48-72 h.When 1 mmol/L GSH was added in the early cultural medium in vitro,it could improve the development efficiency of Yanbian cattle somatic cell cloning embryos,and alleviate oxidative damage from the harmful stress.