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中文摘要：
以疣粒野生稻和栽培稻02428的成熟种子为材料,对愈伤组织的诱导和继代、胚性悬浮细胞系建立、原生质体制备、再生细胞团分化及植株再生进行研究。结果表明：（1）水稻愈伤组织诱导的最佳2,4-D浓度为0.014mmol/L;（2）胚性悬浮细胞系建立的最佳条件为AA悬浮培养基＋0.009mmol/L 2,4-D,每25mL液体培养基加入0.4g愈伤组织的初始接种量,7d的继代周期;（3）原生质体制备的最佳条件为20g/L纤维素酶＋1g/L果胶酶,酶解5h,800r/min离心5min;（4）用荧光增白剂（VBL）细胞壁染色液可以快速、准确的检测原生质体制备及培养过程中细胞壁的变化情况。
英文摘要：
The mature seeds of O. meyeriana and O. Sativa 02428 were used as the materials to study the callus induction and subculture, establishment of embryogenic suspension cells, preparation of protoplasts, differentiation of regeneration of cells and regeneration of plant. The results show that （1） The appropriate concentration of 2,4-D for callus induction is 0. 014 mmol/L. （2） The appropri- ate condition of establishing embryonic suspension cell line is AA suspension medium＋0. 009 mmol/L 2,4-D,0.4 g per 25 mL liquid medium of initial inoculation amount and 7 d of subculture cycle. （3） The appropriate condition of protoplast preparation is 20 g/L Celluase Onozuka RS ＋1 g/L Pectol- yase Y-23 pectinase, 5 h of enzymatic hydrolysis time, 800 r/min of purification speed for 5 min. （4） The method of fluorescent whitening agent （VBL） can be used for rapid and accurate deteetion of cell wall changes in the process of plant protoplast preparation and culture.
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同项目期刊论文
Transcriptomic Analysis and the Expression of Disease-Resistant Genes inOryza meyeriana under Native
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