中文摘要：

目的：研究大鼠脑创伤后外周血及创伤区脑组织中CD34＋细胞数量变化的规律和意义。方法：取雄性Wistar大鼠98只，随机分为创伤组和假手术组，创伤组大鼠制备液压脑创伤模型。每组各抽取7只大鼠，分别于创伤前和创伤后第1、2、3、5和7天取内眦静脉血进行CD34＋细胞计数。伤后第0、1、3、7、14和21天，每组各随机抽取7只大鼠处死，在脑损伤节段取材，采用免疫组化SP染色法检测CD34。结果：与假手术组比较，伤后第1天创伤组大鼠外周血CD34＋细胞计数即显著增加，伤后第2天达到高峰，然后逐渐恢复至正常水平（ F组间＝14．695， F时间＝27．307，F交互＝4．779，P＜0．001）；伤后第1天，创伤区脑组织CD34＋细胞增多，伤后第3天显著增加，伤后第7天达到峰值，随后有所下降，但仍维持在较高水平（F组间＝561．542， F时间＝62．374，F交互＝58．222，P＜0．001）。结论：脑创伤后外周血CD34＋细胞明显动员，并可能归巢到创伤区域，参与血管新生。

英文摘要：

Aim：To detect the changes of CD 34 ＋cells in peripheral blood and in posttraumatic brain tissue of rats with traumatic brain injury（TBI）.Methods：A total of 98 rats were randomly assigned to control and TBI groups ,49 rats in each group .Fluid percussion injury was performed over the right parietal lobe in TBI group .Seven in each group were sampled randomly to collect blood samples from retro-orbital venous plexus before TBI , and at the 1st,2nd,3rd, 5th and 7th day after TBI, and CD34 ＋cells in peripheral blood were evaluated by flow cytometry .At the 1st, 3rd, 7th, 14th, and 21st day after TBI, 7 rats from the 2 groups were sampled respectively and sacrificed to detect CD 34 ＋cells in brain tissue using immunohistochemistry staining .Results：Compared with those of the control group , the number of CD34 ＋cells in peripheral blood began to increase significantly in TBI group at the 1st day after TBI,reached the peak at the 2nd day after TBI,then decreased to the normal level （Fgroup =14.695, Ftime =27.307,Finteraction =4.779,P＆lt;0.001）;the number of CD34 ＋cells in the injured brain tissue began to increase at the 1st day after TBI,and reached the peak at the 7th day after TBI,then decreased gradually , but stabilised at a higher level （ Fgroup =561.542, Ftime =62.374, Finteraction =58.222, P＆lt;0.001）.Conclusion：CD34 ＋cells in peripheral blood are mobilized after TBI ,and maybe home to the injured tissue and play an important role in angiogenesis .