中文摘要：

目的研究透明质酸酶（HAase）与基质金属蛋白酶-9（MMP-9）在人乳腺癌侵袭与转移中的意义。方法应用组织匀浆、酶连吸附测定法及免疫组化等方法测定HAase和MMP-9在人乳腺癌中的表达和其病理特征比较。结果①乳腺癌组织学分级Ⅰ、Ⅱ、Ⅲ级组HAase的分别为4．89±2．55、8．03±2．66、12．00±3．96mU／g（n=10，20，3），Ⅲ级组〉Ⅱ级组〉Ⅰ级组（P〈0．05）。乳腺癌有腋淋巴结转移组HAase表达（8．27±3．26mU／g，n=23）明显高于无腋淋巴结转移组（5．53±2．82mU／g，n=10）（t=-2．305，P=0．028）。②MMP-9阳性率在淋巴结转移组为（78．3％，18／23），明显高于无淋巴结转移组（30．0％，3／10）（P=0．016）。③MMP-9阳性组（n=21）HAase（8．57±3．12）mU／g明显高于阴性组（n=12）（5．47±2．85）mU／g（t=2．830，P=0．008）。结论HAase和MMP-9一样，与人乳腺癌侵袭与转移有关。

英文摘要：

Objective To study the expression of hyaluronidase （HAase） and matrix metalloproteinase-9 （MMP-9 in the invasion and metastasis of human breast cancer. Methods The expression of HAase and MMP-9 in human breast cancer was detected using tissue homogenate preparing, enzyme-linked immunoadsorbent assay （ELSA）, and immunohistochemistry examination. Results ① The levels of HAase were 4.89±2.55, 8.03±2.66, and 12.00±3.96 mU/g in grade Ⅰ, Ⅱ, and Ⅲ of breast cancer, respectively （n = 10, 20, 3）, with significant differences between each other （P 〈 0.05）. The HAase levels were significantly elevated in patients with axillary lymph node metastasis （8.27±3.26 mU/g, n = 23） as compared with patients without metastasis （5.53±2.82 mU/g, n = 10） （t = -2.305, P = 0.028）. ② The positive rate of MMT-9 was significantly higher in patients with axillary lymph node metastasis （78.3%, 18/23） than patients without metastasis （30.0%, 3/10） （P = 0.016）. ③ The expression of HAase was significantly greater in MMT-9 positive patients （8.57±3.12 mU/g, n = 21） than in MMT-9 negative patients （5.47±2.85 mU/g, n = 12） （t = 2.830, P = 0.008）. Conclusions Both HAase and MMT-9 are associated with the invasion and metastasis of human breast cancer.