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Abundance and distribution of ammonia-oxidizing archaea in Tibetan and Yunnan plateau agricultural soils of China
  • 分类:X703[环境科学与工程—环境工程] X141[环境科学与工程—环境科学]
  • 作者机构:[1]Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, China
  • 相关基金:Acknowledgements This study was supported by the National Natural Science Foundation of China (Grant No. 51078207), and Research Fund for the Doctoral Program of High Education of China (No. 20090002770003). We wish to thank the staff of Research Platform for Modem Environmental Biologic Technology of School of Environment in Tsinghua University.
中文摘要:

同样低的氧和高度紫外(紫外) 暴露可能显著地在高原影响微生物引起的存在,它能导致一个专业化微生物引起的社区。在高原的农业土壤决定氧化氨的 archaea (AOA ) 的丰富和分发,七件土壤样品从在栽培小麦,高地大麦,和菜子的西藏和云南的农田分别地是镇定的,它在上面的 32003800 m 的高度被定位海水平。量的 PCR (q-PCR ) 并且克隆图书馆在 amoA 基因上指向被用来确定许多 AOA 和氧化氨的细菌(AOB ) ,并且在样品描绘 AOA 的社区结构。AOA 房间的数字(9.34 敤潶牵摥?

英文摘要:

As low oxygen and high ultraviolet (UV) exposure might significantly affect the microbial existence in plateau, it could lead to a specialized microbial community. To determine the abundance and distribution of ammonia-oxidizing archaea (AOA) in agricultural soil of plateau, seven soil samples were collected respectively from farmlands in Tibet and Yunnan cultivating the wheat, highland-barley, and colza, which are located at altitudes of 3200-3800 m above sea level. Quantitative PCR (q-PCR) and clone library targeting on amoA gene were used to quantify the abundances of AOA and ammonia-oxidizing bacteria (AOB), and characterize the community structures of AOA in the samples. The number of AOA cells (9.34 × 10^7-2.32× 10^8 g^-1 soil) was 3.86-21.84 times greater than that of AOB cells (6.91 × 10^6-1.24 × 10^8 g^-1 soil) in most of the samples, except a soil sample cultivating highland- barley with an AOA/AOB ratio of 0.90. Based Kendall's correlation coefficient, no remarkable correlation between AOA abundance and the environmental factor was observed. Additionally, the diversities of AOA community were affected by total nitrogen and organic matter concentration in soils, suggesting that AOA was probably sensitive to several environmental factors, and could adjust its community structure to adapt to the environmental variation while maintaining its abundance.

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