中文摘要：

目的：观察敲减ALPL基因对人牙髓干细胞（h DPSCs）凋亡的影响。方法：取第3代h DPSCs,将其随机分为实验组和对照组后,通过慢病毒转染法敲减实验组h DPSCs的ALPL基因,并采用Western-Blot及ALP染色法检测两组细胞中ALP蛋白的表达水平。用Western-Blot和流式细胞术分别检测各组h DPSCs的凋亡状态及凋亡相关蛋白caspase-3、caspase-8的表达水平。结果：Western-Blot及ALP染色结果显示,人ALPL敲减病毒颗粒能在h DPSCs中有效敲减ALPL基因;敲减ALPL基因后,可使h DPSCs的凋亡水平及其凋亡相关蛋白caspase-3和caspase-8的表达水平明显上升（P〈0.05）。结论：敲减ALPL基因可促进h DPSCs的凋亡。

英文摘要：

AIM： To investigate the effects of ALPL gene knockdown on the apoptosis of human dental pulp stem cells（ h DPSCs）. METHODS： h DPSCs of the third passage were divided into an experimental group with ALPL gene knockeddown by lentivirus and a control group without gene knockdown. Western blot was performed to detect ALPL protein expression of the cells. Apoptosis of the cells was determined by flow cytometry,and the expression of caspase-3 and caspase-8 was examined by Western Blot. RESULTS： Western blot and ALPL staining results indicated that the ALPL knockdown corresponding virus particles can effectively knock down the expression of ALPL gene in h DPSCs,leading to higher apoptosis of the cells,and higher expression of caspase-3 and caspase-8（ P 〈 0. 05）.CONCLUSION： ALPL knockdown promotes the apoptosis of h DPSCs.