为进一步了解附植藻类对沉水植物的影响,以狐尾藻为研究对象,通过室内模拟实验,研究了有无附植藻类情况下,狐尾藻根长、株长、生物量、可溶性蛋白含量、叶绿素含量、MDA含量、SOD、POD以及CAT等的变化情况.结果表明:未添加藻类处理组中狐尾藻的生物量最大是添加藻类处理组的2.49倍,实验末期未添加藻类处理组的叶绿素含量是添加藻类处理组的1.87倍,说明附植藻类的大量繁殖会影响狐尾藻生物量的合成,降低狐尾藻的叶绿素含量;添加附植藻类处理组的POD和CAT酶活性在实验后期均显著高于无附植藻类的处理组,且实验末期狐尾藻的MDA含量显著上升,说明附植藻类的大量繁殖使狐尾藻细胞膜损害严重,影响了抗氧化保护酶系统,最终导致狐尾藻衰败显著.附植藻类的大量繁殖是沉水植物衰败的原因之一.
In order to further understand the implantation effect of epiphyte on submerged plants, simulated experiment of Myriophyllum Spicatum was carried out. The paper studied the changes of root length, plant length, biomass, soluble protein content, chlorophyll content, MDA content, SOD, POD and CAT. The experimental results showed that the biomass of Myriophyllum Spicatum in the treatment without epiphyte increased hy 2.49 times, and at the end of the experiment, the chlorophyll content increased by 1.87 times. This showed that a large number of breeding epiphyte would affect the synthesis of biomass and decrease chlorophyll content. Enzyme activity of POD and CAT with epiphyte in treatment was higher than the treatment without epiphyte, and MDA content rose substantially at the end of the experiment. It was concluded that a large number of epiphyte damaged cell membrane, affected the antioxidant protective enzyme, and eventually led to significant decline of Myriophyllum Spicatum. Epiphyte is one of the main reasons for decline of submerged plants.