中文摘要：

采用同源克隆和锚定PCR技术，从企鹅珍珠贝Pteria penguin中克隆到热休克蛋白hsp70基因的cDNA全序列。cDNA全长2308bp，3’非编码区域（UTR）为234bp，5’UTR为118bp，开放阅读框（ORF）为1956bp，编码651个氨基酸，分子量为70．97kd，理论等电点为5．24，有2个糖基化位点：NKSI和NVSA，并含有HSP70家族的3个签名序列：IDLGTTYS，DLGGGTFD和IVLVGGSTRIPKIQK，以及C末端的保守序列EE—VD。结合BLAST分析的结果，可以确认获得的cDNA序列为企鹅珍珠贝HSP70的编码序列。与合浦珠母贝Pinctada fucata hsp70的氨基酸序列相比，企鹅珍珠贝多1个糖基化位点NVSA，少1个氨基酸，包括1个氨基酸插入和2个缺失。两者的氨基酸序列一致性高达92％，突变位点52个；两者的核苷酸序列一致性高达79％，突变位点416个。系统发育分析表明两者的亲缘关系最近，与传统分类相符，然后与太平洋牡蛎等聚合在一起。 该基因的克隆和比较分析为进一步深入研究珍珠贝类的抗逆机理、抗性育种及其进化具有重要意义。

英文摘要：

The heat shock protein 70 gene （hsp70） from penguin pearl oyster Pteria penguin was cloned using techniques of homological cloning and anchored PCR. The full length of eDNA sequence is 2 308 bp, containing a 3＇UTR （untranslated region） of 234 bp, a 5＇UTR of 118 bp, and an ORF of 1 956 bp which encodes a polypeptide of 651 amino acids with an estimated molecular weight of 70.97 kd. There are two glycosylation sites, NKSI and NVSA. Three signature sequences of heat shock protein 70 family （HSP70 family）, IDLGTTYS, DLGGGTFD and IVLVGGSTRIPKIQK, were detected in the deduced amino acid sequence. These results, together with blast analysis, indicate that the eDNA sequence cloned in this study is a member of heat shock protein 70 family. Compared with Hepu pearl oyster Pinctada fucata＇s hsp70 gene, penguin pearl oyster has an amino acid reduced due to an insertion and two deletions of amino acids. The identities between these two pearl oysters are 92% and 79% for amino acid and nueleotide sequences, respectively. Phylogenetie analysis shows that the two pearl oyster species are most closely related, supporting the traditional classification. It is also indicated that the pearl oysters are closely related with Crassostrea oysters instead of scallops. Cloning and comparison of hsp70 genes from different pearl oyster species could be useful in the studies of anti-stress mechanism, tolerance-improvement breeding and evolution for these animals.