中文摘要：

为了解翘嘴鳜胚胎发育中miR-146a的表达规律以及温度对miR-146a表达的影响,将翘嘴鳜受精卵分别在22℃和25℃水温下孵化,并利用实时荧光定量方法检测miR-146a在各个发育阶段的表达.结果显示,在22℃孵化时,miR-146a的表达在原肠期前处于较高水平且各时期表达没有显著性差异（P〉0.05）,在尾芽期后出现显著性降低（P〈0.05）,并在出膜时达到最低值;在25℃孵化时,miR-146a的表达直到神经胚期仍处于较高水平且各时期表达没有显著性差异（P〉0.05）,发育进入尾芽期后表达显著降低（P〈0.05）,在肌效期达到最低值;miR-146a在25℃水温孵化时的表达较22℃孵化同时期的表达都出现显著降低（P〈0.05）.研究表明,miR-146a可能主要在胚胎发育后期起到调控胚胎发育的作用,在尾芽期后通过下调表达来促进胚胎的发育;并且温度能影响miR-146a的表达,温度升高会降低miR-146a在胚胎发育各个时期的表达,将有利于胚胎的发育.

英文摘要：

To investigate the expression pattern of miR-146 a in embryonic developmental stage and the effect of temperature on the expression of miR-146 a in Siniperca chuatsi,the embryos were incubated under different temperatures and the expression of miR-146 a during embryonic developmental stage was detected by RT-q PCR. The results showed that the expressions of miR-146 a were at a higher level and there is no significant difference before gastrula stage when the embryos incubated under 22 ℃（ P〉0. 05）. And then the expressions of miR-146 a decreased after tail-bud stage and decreased to the lowest level at larval stage（ P〈0. 05）. When the embryos incubated under 25 ℃,the expressions of miR-146 a were at a higher level and there is no significant difference till neurula stage（ P〉0. 05）. Once at tail-bud stage,the expression of miR-146 a significantly decreased（ P〈0. 05） and to the lowest level at muscular effect stage. It was interesting that the expressions of miR-146 a were significantly decreased at each developmental stage incubated under 25 ℃ when compared with 22 ℃ incubation（ P〈0. 05）. The results indicated that miR-146 a mainly involved in regulating embryonic development during later embryonic developmental stages and promoted embryonic development by down regulation its expression. In addition,the expressions of miR-146 a could decrease when the incubation temperature increased,and miR-146 a might involve in the effect of temperature on embryonic development of Siniperca chuatsi.