中文摘要：

探讨西兰花中异硫氰酸盐（isothiocyanates,ITCS）诱导人胃腺癌SGC-7901细胞凋亡过程中的作用及其可能机制．不同质量浓度ITCS处理人胃腺癌SGC-7901细胞。通过SRB法、细胞形态学观察、流式细胞仪和激光共聚焦显微镜实验。观察ITCS对SGC-7901细胞的抑制率，对细胞凋亡的影响．ITCS可抑制SGC-7901细胞增殖；细胞出现早期凋亡细胞的形态；0、60、120、240μg／mL的ITCS作用于SGC-7901细胞48h后。可见细胞凋亡率分别为（4．3±1．6）％、（9．1±3．8）％、（20．1±4．2）％和（55．4±4．9）％；细胞内的Ca^2＋浓度升高．ITCS能够促进人胃腺癌SGC-7901细胞的凋亡，其作用机制可能是细胞内Ca^2＋浓度的升高．

英文摘要：

To investigate the pro-apoptosis effect of isothiocyanates （ITCS） on human gastric adenoma cells SGC -7901 and its possible mechanism. SGC- 7901 cells are treated with different concentrations of ITCS. Through SRB assay, fluorescence microscope, flow cytometry and laser confocal microscopy to see the inhibitory rate, apoptosis and cell cycle of SGC - 7901 cells by ITCS. ITCS inhibited proliferation of SGC -7901 cells; cells appeared the modality of early apoptosis; 300,600,1200μ g/mL of ITCS affected on SGC -7901 cells for 48 h,the apoptosis is （4. 3 ± 1.6）%, （9. 1 ±3. 8）%, （20. 1 ±4. 2）% and （55.4 ±4. 9）% ; the variation of Ca^2＋ in cells is increased. ITCS could promote the apoptosis of SGC -7901 cells, the mechanism should be that ITCS could increase the Ca^2＋ in SGC -7901.