中文摘要：

目的 探讨高钠盐诱导心脏成纤维细胞（CF）增殖的作用机制及石斛碱的干预效应。方法 体外组织块贴壁法培养大鼠CF,实验分为3组：对照组（139 mmol/L Na＋）、高钠盐组（161 mmol/L Na＋）、石斛碱组（161 mmol/L Na＋＋10-5mol/L石斛碱）,培养48 h。CCK-8细胞增殖试剂盒与MTT比色法检测各组细胞增殖情况;Western blot检测细胞增殖细胞核抗原（PCNA）、磷酸化细胞外调节蛋白激酶1/2（p-ERK1/2）、磷酸化核因子κBp65（p-NF-κB p65）蛋白表达水平;实时荧光定量PCR检测细胞单核细胞趋化蛋白1（MCP-1）、血管细胞黏附分子1（VCAM-1）、细胞间黏附分子1（ICAM-1）mRNA表达水平。结果 与对照组比较,高钠盐组培养48 h后,CF增殖明显增加;与高钠盐组相比,10-5mol/L石斛碱显著抑制CF增殖。与对照组相比,高钠盐组CF中p-ERK1/2、p-NF-κBp65蛋白表达增高,炎性因子MCP-1、VCAM-1和ICAM-1 mRNA表达增加;与高钠盐组相比,石斛碱可明显降低PC-NA、p-NF-κB p65蛋白表达及MCP-1 mRNA表达,但对p-ERK1/2、VCAM-1和ICAM-1表达无明显影响。结论 高钠盐诱导CF增殖及炎性因子表达,其机制与p-ERK1/2、p-NF-κB p65表达上调有关;石斛碱抑制上述效应,其机制与抑制p-NF-κB p65有关。

英文摘要：

Aim To investigate the mechanism of high sodium salt induced proliferation of cardiac fibroblasts （CFs） and the intervention effect of dendrobine. Methods Rat CFs were cultured with tissue explant method in vitro. The experiments were divided into 3 groups： control group （containing 139 mmol/L Na＋）, high sodium salt group （containing 161 mmol/L Na＋） , dendrobine group （ 161 mmol/L Na＋ 10^-5 mol/L dendrobine） , and cells were cultured for 48 hours. CCK-8 cell proliferation kit and MTr colorimetric assay were used to detect the proliferation of cells in each group. The protein expressions of proliferating cell nuclear antigen （ PCNA）, phosphorylated extracellular regulated protein kinase 1/2 （p-ERK1/2） and phosphorylated nuclear factor KB p65 （p-NF-gB p65） were detected by Western blot assay. The mRNA expressions of monocyte chemotactic protein 1 （MCP-1）, vascular cell adhesion molecule 1 （VCAM-1） and inter- cellular adhesion molecule-1 （ICAM-1） were detected by real-time fluorescence quantitative PCR. Results Compared with the control group, the proliferations of CFs in the high sodium salt group were significantly increased after 48 hours culture. Compared with the high sodium salt group, 10-5 mol/L dendrobine could significantly inhibit the proliferation of CFs. Compared with the control group, the protein expressions of p-ERK1/2 and p-NF-KB p65 were increased, inflammatory factors MCP-1, VCAM-1 and ICAM-1 mRNA expressions were increased in high sodium salt group. Compared with the high sodium salt group, dendrobine could significantly reduce the expressions of PCNA and p-NF-KB p65 proteins and the expression of MCP-1 mRNA, but had no significant effect on the expressions of p-ERK1/2, VCAM-1 and ICAM-1. Conclusion High sodium salt induces the proliferation of CFs and the expression of inflammatory factors, and its mecha-nism is related to the upregulation of p-ERK1/2 and p-NF-KB p65 expressions. Dendrobine inhibits these effects and its mechanism is related to the