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中文摘要：
建立了一种基于生物质谱的乙酸酐稳定同位素标记，定量蛋白质组学研究方法，优化了影响标记效率的各种条件。在pH8．0的Na2B4O7／H3BO3缓冲体系中，当乙酸酐摩尔浓度25倍过量于肽段摩尔量，22℃反应30min时，标记即可完全。对多对H6／D6-乙酸酐标记肽段在基质辅助激光解吸电离质谱中的动态范围及定量准确度进行了考察，并通过串联质谱分析确定了乙酰化位点。结果表明：在10倍和30倍动态范围内，线性关系良好（r=0．99，r=0．98），理论值和观测值的偏差分别为0．5％和20％。
英文摘要：
A strategy for quantitative proteome analysis was established through H6/D6-acetic anhydride stable isotopic labeling. Several major influencing factors, such as reactive buffer system, acetic anhydride concentration, pH value, temperature and reaction time, were examined. The results showed that a satisfied acetic anhydride labeling could be realized in a pH 8.0 reactive buffer composed of Na2B4OT/H3BO4 when the reaction was kept for 30 minutes at 22℃, with a 25-fold molar excess of acetic anhydride over free amino groupscontained peptides. Under the optimized conditions, the efficiency of acetic anhydride labeling for the tryptic peptides was up to 100%. The investigation on the dynamic range and quantitative accuracy were performed by calculating the ratio of the peak intensity in mass spectra with several H6/D6-acetylated peptide couples. A good linearity was obtained within the dynamic ranges of 1：10 and 1：30, with the R^2value of 0.99 and 0.98 and RSD of 0.5% and 20% , respectively.
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