中文摘要：

目的 研究核仁素核酸适配体（AS1411）介导信号转导与转录激活因子3（signal transduction and activator of transcription 3,STAT3）反义寡核苷酸（antisense oligonucleotide,ASO）靶向抗肿瘤的作用。方法以RNA为耦联分子,连接靶向分子AS1411和效应分子ASO。以RNA Structure软件对预合成的不同RNA碱基耦联构建的嵌合体分子二级结构进行预测分析。以琼脂糖凝胶电泳检测嵌合体分子在血清及细胞裂解液中的稳定性。通过流式细胞术和荧光共聚焦实验检测AS1411介导STAT3 ASO进入肿瘤细胞的情况。通过CCK-8试剂盒检测STAT3 ASO对肿瘤细胞生长的抑制情况。通过RT-PCR和Western blot分析ASO对肿瘤生长相关基因表达的影响。结果AS1411可介导STAT3 ASO高效进入肿瘤细胞,抑制C-myc、Cyclin D1、Bcl-xl和PD-L1基因的转录和翻译,并能抑制Du145细胞的生长。结论 AS1411可介导STAT3 ASO靶向进入肿瘤细胞,从而发挥抗肿瘤的作用。

英文摘要：

Objective To observe the effect of AS1411-mediated signal transduction and activator of transcription 3 （STAT3） antisense oligonucleotide （ASO） targeting tumor cells. Methods RNA was used as coupling molecules to link the targeting molecules AS1411 and effector molecules ASO. Prediction and analysis of the secondary structure of the pre-synthesis of chimeric molecules by RNA Structure software. Agarose gel electrophoresis was used to test the stability of chimeric molecules in serum and cell lysis solution. Using flow cytometry and confocal fluorescence microscope were used to estimate the internalization of AS1411-mediated STAT3 ASO. Inhibitory effect of ASO on the growth of tumor cells was detected by CCK-8 kit. RT-PCR and Western blot was used to measure the expression levels of tumor related genes. Results STAT3 ASOs mediated by AS1411 can enter tumor cells efficiently to inhibit the transcription and translation of C-myc, Cyclin D1, Bcbxl and PD-L1 gene,and also can inhibit the growth of Du145 cells. Conclusions AS1411-mediated STAT3 ASO can enter tumor cells and act as anti-tumor medicine.