中文摘要：

目的探讨炎性因子TNF-α是否参与调控半胱氨酰白三烯受体1（CysLTl）在支气管上皮细胞的表达。方法于细胞培养液中加入不同质量浓度（0．00、0．05、0．50、5．00、20．00μg／L）的TNF-α仅刺激细胞支气管上皮细胞株16HBE48h，采用反转录（RT）-PCR法检测CysLT1 mRNA的水平，免疫组织化学染色检测CysLT1 在16HBE细胞的表达。结果免疫组织化学染色法在16HBE细胞未能检测到CysLTl，但用TNF-α仅处理细胞48h，可检测到CysLTl明显表达。RT—PCR法在16HBE细胞能检测到微弱的CysLT1 mRNA带，TNF-α刺激细胞48h后，CysLT1 mRNA表达增强。当TNF-α质量浓度分别为0．00、0．05、0．50、5．00、20．00μg／L时，CysLT1 mRNA／8．actin相对密度比分别为0．048、0．105、0．177、0．182、0．495。结论TNF-d能上调CysLT1在支气管上皮细胞株16HBE细胞的表达，这种上调作用呈浓度依赖性。在呼吸道病毒感染时，局部产生大量的TNF-α上调呼吸道上皮细胞CysLT1表达，增强呼吸道的炎性效应，这可能是病毒性呼吸道感染时诱发喘息发作的机制之一。

英文摘要：

Objective To explore whether TNF-α involves in the modulation of Cysteinyl leukotriene receptor 1 （CysLT1） expression in bronchial epithelial cells. Methods The bronchial epithelial cell lines 16HBE cells were stimulated with different concentration （0.00,0.05,0.50,5.00,20. O0 μg/L） of TNF-α for 48 hours, and CysLT1 mRNA in 16HBE cells was measured by reverse transcription（RT）-PCR. CysLT1 expression was detected by immunohistochemistry. Results 16HBE cells did not express CysLT1 ,after the cells were treated with TNF-α,obvious expression of CysLT1 were detected by immunohistochemistry. The weak CysLT1 mRNA expression was observed by RT-PCR in 16HBE cells, and after the cells were treated with TNF-α for 48 hours, CysLT1 mRNA expression were upregulated. When the concentrations of TNF-α were 0.00,0.05,0.50,5.00, and 20.00 μ/L respectively, the relative intensities of CysLT1 mRNA/β-actin were 0. 048,0. 105,0. 177,0. 182,0. 495 ,respectively. Conclusions TNF-α can upregulate CysLT1 mRNA expression in 16HBE cells in a dose-dependent manner. When infected by virus, respiratory tract produces abundant TNF-α. The TNF-α can upregulate the expression of CysLT1 in epithelial cells, enhance inflammation reaction in respiratory tract. This may explain partially the mechanism of exacerbation of asthma induced by respiratory tract infection.