中文摘要：

以课题组前期筛选得到的菌株Tolumonas osonensis P2-A-1为对象,通过菌体初始浓度、底物种类和浓度、阳极液p H、外电阻等参数的优化以及细胞的通透化处理,提高其在微生物燃料电池中的产电性能。结果表明,菌株T.osonensis P2-A-1接种到TSB液体培养基中,22℃有氧条件下静置培养48 h（OD600=2.714）,离心收集的菌体经1mmol·L^-1 EDTA处理20 min后,接种到阳极液Ⅱ（PBBM液体培养基中加入终浓度为2.000 g·L^-1的乙酸钠（作为底物）和终浓度为5.000 g·L^-1的柠檬酸铁,p H 7.2）,制备得到细胞浓度为3.394 g·L^-1的接种液,注入MFCs反应器,外接1000Ω电阻,于室温下运行14 d,功率密度达到509 m W·m^-2,比优化前提高了162.4%。这是首次对T.osonensisa种内微生物产电性能及其在微生物燃料电池中应用的报道。其成果对于丰富产电微生物的多样性,挖掘更多具有高电化学活性的微生物纯种、提高其产电性能具有重要的指导意义。

英文摘要：

Power generation performance of Tolumonas osonensis P2-A-1 in MFCs was improved by optimizing initial cell concentration in inoculation medium, substrate type and concentration, anolyte p H, external resistance, and chemical solvent permeabilization. The optimized experimental condition is inoculation in TSB liquid medium and statically incubated at 22℃ under aerobic conditions. OD600 of the culture was 2.714 after 48 h of incubation and cells were harvested by centrifugation and treated by 1 mmol·L^-1 EDTA for 20 min. The cells were added into anolyte Ⅱ（PBBM liquid medium containing 2.000 g·L^-1 sodium acetate as the substrate and 5.000 g·L^-1 ferric citrate, p H 7.2） to prepare inoculation media with dry cell concentration of 3.394 g·L^-1. After 14 d at room temperature, the power density of MFCs connecting to the external resistance 1000 Ω reaches to 509 mW ·m^-2, which is increased by 162.4% compared with the original. It is the first time that T. osonensis is applied in MFCs and shows electrochemical activity.