中文摘要：

目的 研究补阳还五汤抗同型半胱氨酸（Hcy）诱导的脐静脉内皮细胞（HUVEC）损伤的作用及机制。方法 以补阳还五汤连续灌胃SD大鼠1周,提取补阳还五汤含药血清。将HUVEC置于5%CO2、37℃细胞恒温培养箱中培养,当细胞汇合度达到80%,选取对数期细胞,开始分组与模型制备。将HUVEC随机分为10组：空白组（10%空白血清）、模型组（10%空白血清＋Hcy）、小中大3个百分含量实验组（5%,10%,20%补阳还五汤）、模型组-Ⅰ（10%胎牛血清＋Hcy）、抑制剂-Ⅰ组（10μmol·L-1MAPK抑制剂SB203580＋Hcy）、抑制剂-Ⅱ组（10μmol·L-1ERK抑制剂PD98059＋Hcy）、抑制剂-Ⅲ组（10μmol·L-1Rho激酶抑制剂Y27632＋Hcy）、抑制剂-Ⅳ组（10μmol·L-1MLCK抑制剂ML-7＋Hcy）,且Hcy造模浓度为3 mmol·L-1。用噻唑蓝（MTT）测定Hcy对HUVEC的毒性作用;用蛋白质印迹法测定丝裂原活化蛋白激酶（p38MAPK）、细胞外调节蛋白激酶（ERK1/2）蛋白的表达量;用MillicellERS电阻仪测定各组单层细胞跨膜电阻值（TEER）的变化。结果 与空白组的光密度值（代表细胞增值）为0.95±0.05比较,7个Hcy梯度组的光密度值分别是0.91±0.06,0.88±0.08,0.68±0.06,0.60±0.07,0.58±0.04,0.51＋0.06,0.40＋0.02,差异有统计学意义（P〈0.05）。P38MAPK蛋白的相对表达量：空白组、模型组、小中大3个百分含量实验组、模型对照组（即模型-Ⅰ组）及4个抑制剂组分别是0.42±0.06,1.10±0.12,0.33±0.03,0.68±0.08,0.46±0.04,0.42±0.03,0.37±0.04,0.48±0.05,0.32±0.02,0.63±0.08。磷酸化P38MAPK蛋白相对表达量以上各组分别是0.12±0.01,0.42±0.04,0.40±0.05,0.18±0.02,0.20±0.01,0.22±0.02,0.18±0.02,0.30±0.03,0.17±0.01,0.19±0.02。与空白组比较,模型组的P38MAPK、磷酸化P38MAPK表达量显著增加,差异有明显统计学意义（P〈0.01）。与模型组比较,中高2个百分含量实验组和抑制剂组,磷酸化P38MAPK蛋白相对表达量表达显著下降,差异

英文摘要：

Objective To explore the protective effect and its mechanism of Buyang Huanwu Tang（BHT） on human umbilical vein endothelial cells （HUVEC） injuries by homocysteine （Hcy） . Methods The BHT was used lavage SD rats a week in a row, extracting serum containing BHT. HUVEC were cultured at 37 ℃, 5% CO2 cell culture incuba- tor. When cells convergence degree reached 80% , selecting logarithmic phase cells began to divided group. HUVEC were induced by Hey and randomly divided into 10 groups： blank group（ 10% blank serum） ,model group（10% blank serum ＋ Hcy）, low, middle and high doses experimental groups （5%, 10%, 20% BHT, respectively）, model- Ⅰ group（ 10% fetal bovine serum ＋ Hcy）, inhibitor - Ⅰ （ 10 μmol .L-1MAPK inhibitor SB203580 ＋ Hey）, inhibitor - Ⅱ （ 10 μmol .L-1 ERK inhibitor PD98059 ＋ Hey） , inhibitor -Ⅲ （ 10μ mol . L-1 Rho kinase inhibitor Y27632 ＋ Hcy）, inhibitor -Ⅳ group（10 μmol . L-1MLCK inhibitor ML-7 ＋Hcy）. Hcy concentration was 3 mmol . L-1, and each inhibitor concentration was 10 μmol . L-1 . The toxic effects of Hcy on HUVEC was determined by MTr kit. The hanges of protein level about p38 mitogen activated protein kinase （MAPK）, external -signal regulated kinase （ERK1/2） were determined by Western blot. Meanwhile, Millieell -ERS resistance tester is used to test the change of groups trans epithelial electric resistance （TEER）. Results The Optical density in normal group was （0.95 ±0. 05） and optical density in Hcy gradient groups were （ 0.91 ± 0. 06 ）, （ 0. 88 ± 0. 08 ）, （ 0. 68 ± 0. 06 ）, （ 0. 60 ± 0.07 ）, （0. 58 ±0. 04）, （0. 51 ±0. 06）, （0. 40 ±0. 02） respectively with statistical difference（P 〈0. 05）. The p38MAPK rela- tive expression in blank group, model group,low, middle and high doses experimental groups, model - Ⅰ group, inhibi- tor - Ⅰ , inhibitor - Ⅱ , inhibitor -Ⅲ, inhibitor - Ⅳ group （ ten groups ） were （ 0. 42± 0. 06 ）, （ 1.10 ± 0. 12 ）