中文摘要：

利用逆转录多聚酶链式反应（RT—PCR）分析癌基因HDM2在人宫颈癌Hela细胞中的表达.对克隆到的HDM2基因片段进行测序分析,从中筛选到一种新的HDM2剪接变异体.该剪接变异体阅读框由1401bp组成,预测编码466个氨基酸.与野生型HDM2相比,该变异体氨基酸序列的29-53位缺失,395位丝氨酸突变为苯丙氨酸,407位丝氨酸突变为半胱氨酸,其中缺失段29-53位于HDM2与p53相结合区域的上游部分.这可能影响HDM2与p53的相互作用,与p53的失活及癌细胞的转移相关.

英文摘要：

Through RT-PCR analysis of the expression about the HDM2 protein in Hela cells,a new HDM2 splice variant was identified.It possessed 1 401 bp reading frame which was predicted to code 466 amino acids,generating an amino acidic loss from residue R29 to V53 and two point mutations（S395F and S407C） compared with wild HDM2.This splice variant,which was located in upstream of the p53 binding site,may be responsible for the inactivation of p53 and tumor cells transforming pathway.