目的研究簇花清风藤Sabia fasciculata枝叶的化学成分。方法采用硅胶柱色谱、SephadexLH.20、制备液相等多种色谱技术进行分离纯化,根据理化性质和波谱数据鉴定化合物的结构。结果从簇花清风藤枝叶95%乙醇提取物中分离鉴定了15个化合物,其中7个五环三萜类化合物:3-氧代-12-烯-28-齐墩果酸甲酯(1)、白桦脂醇(2)、3-氧-△11,13(18)齐墩果二烯(3)、齐墩果酸(4)、imbericacid(5)、拟人参皂苷RP,(6)、竹节参苷IVa(7);3个黄酮类化合物:槲皮素(8)、芦丁(9)、mutabiloside(10);3个生物碱:5.氧阿朴啡碱(11)、N-p-阿魏酰酪胺(12)、N-反式香豆酰酪胺(13);2个甾体类化合物:β-谷甾醇(14)、β-胡萝卜苷(15)。结论所有化合物均为首次从该植物中分离得到,化合物1、5、6、7、10为首次从该属植物中分离得到。
Objective To investigate the chemical constituents in the dried leaves of Sabia fasciculata. Methods Column chromatography such as silica gel, Sephadex LH-20, and preparative HPLC were used to isolate and purify the compounds. Spectroscopic methods like MS, 1H-NMR and 13C-NMR, and physical constants were used to elucidate their structures. Results Fifteen compounds were isolated from 95% ethanol extracts of S. fasciculata, including seven pentacyclic triterpenoids, such as methyl-3-oxo-olean-12-ene-28-oate (1), betulin (2), 3-oxo-olean-ATM 130S)-diene (3), oleanolic acid (4), imberic acid (5), pseudo- ginsenoside RP1 (6), and chikusetsusaponin IVa (7); three flavonoids, such as quercetin (8), rutin (9), and mutabiloside (10); three alkaloids, such as fuseine (11), N-p-feruloyltyramine (12), and N-trans-coumaroyl tyramine (13); two steroids, such as β-sitosterol (14) and β-daucosterol (15). Conclusion All the compounds are isolated from the plant for the first time. Compounds 1, 5--7, and 10 are isolated from the plants ofSabia Colelbr. for the first time.