中文摘要：

以＂五彩珍珠＂番茄为试材,选取6d苗龄无菌苗的子叶和下胚轴为外植体,研究了不同激素浓度及配比对番茄愈伤组织的诱导、不定芽分化及生根的影响,以建立番茄再生体系。结果表明：诱导＂五彩珍珠＂番茄愈伤组织的最佳培养基为MS＋0.4mg·L~（-1）IAA＋2.0mg·L~（-1）6-BA,其子叶的诱导率为100%,下胚轴的诱导率为95%;不定芽分化的最佳培养基为MS＋0.2mg·L~（-1） IAA＋（1.0~3.0）mg·L~（-1） 6-BA;诱导生根的最佳培养基为1/2MS＋0.10mg·L~（-1）NAA。子叶为最佳外植体,其不定芽的分化率及生长情况均优于下胚轴。

英文摘要：

＇Colorful pearl＇ tomato was used as the test material,cotyledon and hypocotyledonary axis of 6day-old sterile seedlings were used as explants to study the effect of different hormone concentration and hormone ratio on induction of tomato callus,differentiation of adventitious buds and rooting induction in order to establish regeneration system for tomato.The results showed that the optimum culture medium for induction of tomato callus was MS＋0.4 mg·L~（-1）IAA＋2.0 mg·L~（-1） 6-BA,in which the induction rate of cotyledon and hypocotyledonary axis was 100%and 95%,respectively.The optimum culture medium for differentiation of adventitious buds was MS＋0.2mg·L~（-1） IAA＋（1.0-3.0）mg·L~（-1） 6-BA;the optimum culture medium for rooting induction was 1/2MS＋0.10mg·L~（-1） NAA.It concluded that cotyledon was the optimal explant.Its adventitious bud differentiation and growth rates were better than those of hypocotyledonary axis.