在实验条件下,将健康性成熟雄性长江华溪蟹Sinopotamon yangtsekiense暴露于0、7.25、14.5、29、58和116mg/L浓度的镉(Cd2+)溶液中,分别于1d、3d、5d和7d时测定精巢中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性及脂质过氧化产物丙二醛(MDA)的含量。结果显示,不同时间段3种酶活性和MDA含量均具有浓度和时间效应关系,表明急性镉暴露对精巢有明显的毒性作用,其作用机制与抗氧化酶活力变化和脂质过氧化加剧有关。
To investigate the reproductive toxicity and mechanisms of acute cadmium(Cd) exposure in the freshwater crab Sinopotamon yangtsekiense,the crabs were exposed to 0,7.25,14.5,29,58 and 116 mg/L of Cd2+ for 1 d,3 d,5 d and 7 d and the activities of superoxide dismutase(SOD),guaiacol peroxidase(GSH-Px),catalase(CAT) were determined,as well as the content of malonyldialdehyde(MDA) in the spermaries.The results showed that the activities of SOD,CAT and GSH-Px were increased initially and decreased subsequently with the increasing concentration of Cd2+ and exposure periods.However,the content of MDA was continuatively increased.These results suggest that Cd is clearly toxic on spermary in the freshwater crab,and the mechanism might be associated with the changes of antioxidant enzyme activities and the increased lipid peroxidation.