ObjectiveTo 学习 clemastanin B (CB ) 的抗病毒的活动, epigoitrin, phenylpropanoids 部分(锐气) 和从 Banlangen (根值 Isatidis ) 的 phenylpropanoids,碱和器官的酸部分(锐气 +ALK + OA ) 的混合 .MethodsThe 实验由四部分组成了:治疗学的行动, prophylaxsis 行动,病毒附件的抑制,和直接 virucidal 行动。 Cytopathic 效果(用户终端设备)和3-( 4,5-Dimethylthiazol-2-yl ) -2,5-diphenyltetrazolium ( MTT )被用来估计抗病毒的 activity.ResultsCB , epigoitrin ,锐气和从 Banlangen (根值 Isatidis )的部分显著地提取的锐气+ALK + OA 增加了 MDCK 房间的生存能力在所有冲淡与病毒控制组相比与病毒预先感染( P < 0.01 )。有 Banlangen (根值 Isatidis ) 的部分显著地在所有冲淡提取的纯混合物或化学药品的 Pretreated 改进了 MDCK 房间的生存能力(P < 0.01 ) 。到由 CB, epigoitrin 和锐气的宿主细胞的病毒吸收的抑制在剂量依赖者 manner.ConclusionCB, epigoitrin,锐气和锐气 +ALK + OA 由禁止病毒增加施加他们的反流行性感冒活动, prophylaxsis 并且堵住病毒附件。锐气和锐气 +ALK + OA 的行动的主要模式是病毒复制的抑制。病毒附件和增加上的禁止的效果是为 epigoitrin 的主要模式。
OBJECTIVE: To study the antiviral activities of clemastanin B(CB), epigoitrin, phenylpropanoids portion(PEP) and the mixture of phenylpropanoids, alkaloids and organic acid fractions(PEP+ALK+OA)from Banlangen(Radix Isatidis).METHODS: The experiment consisted of four parts:therapeutic action, prophylaxsis action, inhibition of virus attachment, and direct virucidal action. Cytopathic effect(CPE) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium(MTT) were used to assess antiviral activity.RESULTS: CB, epigoitrin, PEP and PEP + ALK + OA fractions from Banlangen(Radix Isatidis) extract significantly increased the viability of MDCK cells pre-infected with the virus compared with the virus control group in all the dilutions(P 0.01). Pretreated with either pure compounds or chemical frac-tions of Banlangen(Radix Isatidis) extract in all the dilutions significantly improved the viability of MDCK cells(P 0.01). The inhibition of virus absorption to the host cells by CB, epigoitrin and PEP was in a dose dependent manner.CONCLUSION: CB, epigoitrin, PEP and PEP+ALK+OA exert their anti-influenza activity by inhibiting the virus multiplication, prophylaxsis and blocking the virus attachment. The primary mode of action of PEP and PEP + ALK + OA is the inhibition of virus replication. The inhibitory effects on virus attachment and multiplication are the main modes for epigoitrin.