中文摘要：

易碎的 X 智力迟钝蛋白质(FMRP ) 的缺席引起易碎的 X 症候群(FXS ) ，它是世袭智力迟钝的领先的原因。易碎的 X 相关的蛋白质 1 (FXR1P ) ，它在正常肌肉开发起一个重要作用，是 FMRP 的二正染色体的 paralogs 之一。理解 FXR1P 的函数，我们由使用酵母屏蔽了交往 FXR1P 蛋白质二混血儿的系统。易碎的 X 相关的基因(FXR1 ) 1 被熔化到 pGBKT7 然后用作诱饵屏蔽人的胎儿的大脑 cDNA 图书馆。屏蔽结果揭示了包括 Bcl-2-associated 抄写因素 1 的 10 交往 FXR1P 蛋白质(BTF ) 。在 FXR1P 和 BTF 之间的相互作用被使用在选择媒介的牛乳糖试金和生长测试证实。在哺乳动物的房间的 Co-immunoprecipitation 试金也被执行证实 FXR1P/BTF 相互作用。而且，我们在老鼠在原子核附近在细胞质证实了与 FXR1P co 局部性的那 BTF 由使用共焦的荧光显微镜学的脉管的光滑的肌肉房间。这些结果提供线索阐明在 FXR1P 和 FXS 之间的关系。

英文摘要：

The absence of fragile X mental retardation protein （FMRP） causes fragile X syndrome （FXS）, which is the leading cause of hereditary mental retardation. Fragile X-related protein 1 （FXR1P）, which plays an important role in normal muscle development, is one of the two auto- somal paralogs of FMRP. To understand the functions of FXR1P, we screened FXR1P-interacting proteins by using a yeast two-hybrid system. The fragile X-related gene 1 （FXR1） was fused to pGBKT7 and then used as the bait to screen the human fetal brain cDNA library. The screening results revealed 10 FXR1P-interacting proteins including Bcl-2-associated transcription factor 1 （BTF）. The inter- action between FXR1P and BTF was confirmed by using both β-galactosidase assay and growth test in selective media. Co-immunoprecipitation assay in mammalian cells was also carried out to confirm the FXR1P/BTF inter- action. Moreover, we confirmed that BTF co-localized with FXR1P in the cytoplasm around the nucleus in rat vascular smooth muscle cells by using confocal fluorescence micros- copy. These results provide clues to elucidate the relation- ship between FXR1P and FXS.