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亚致死浓度氯虫苯甲酰胺对小菜蛾药剂敏感度和解毒酶活性的影响

ISSN号：1008-7303
期刊名称：农药学学报
时间：0
页码：464-470
分类：S481.1[农业科学—农药学;农业科学—植物保护] S482.3[农业科学—农药学;农业科学—植物保护]
作者机构：[1]中国农业大学昆虫学系,北京100193
相关基金：国家自然科学基金项目（30971941 30671389）; 公益性农业行业专项课题（201103021）
相关项目：呋喃虫酰肼抗性小菜蛾中EcR/USP基因表达及功能分析

关键词：氯虫苯甲酰胺, 小菜蛾, 亚致死浓度, 敏感度, 解毒酶, chlorantraniliprole, Plutella xylostella, sublethal concentrations, susceptibility, detoxifying enzymes

中文摘要：

采用叶片药膜法,使用亚致死浓度（LC10、LC25）的氯虫苯甲酰胺对小菜蛾Plutella xylostella（L.）3龄幼虫连续处理5代后,试虫对氯虫苯甲酰胺的敏感度分别比敏感品系下降了57.3%和67.7%,同时对多杀菌素的敏感度也分别下降了60.2%和51.5%,但对毒死蜱和高效氯氰菊酯的敏感度变化不明显。采用该浓度的氯虫苯甲酰胺分别处理小菜蛾3龄幼虫24、48和72 h,可诱导其羧酸酯酶（CarE）比活力上升,但对细胞色素P450 O-脱乙基酶（ECOD）、谷胱甘肽S-转移酶（GSTs）和芳基酰胺酶（AA）有明显的抑制作用;连续处理5代后,小菜蛾CarE和ECOD的比活力显著高于对照组,分别为对照组的1.16、1.40倍和1.65、1.56倍,但GSTs和AA的比活力则分别比对照下降了11.0%、27.5%和43.6%、52.5%。结果表明,小菜蛾对氯虫苯甲酰胺产生抗性的风险较高;羧酸酯酶和多功能氧化酶可能与小菜蛾对氯虫苯甲酰胺的敏感度下降有关。

英文摘要：

By using the leaf-dip method,effects of sublethal concentrations of chlorantraniliprole on insecticide susceptibility and detoxifying enzyme activity in the larvae of diamondback moth（DBM）,Plutella xylostella,were investigated.After continuous treatment with sublethal concentrations（LC10 and LC25） of chlorantraniliprole for five generations,the susceptibility of DBM to chlorantraniliprole and spinosad decreased by 57.3%,67.7% and 60.2%,51.5% respectively,compared to the untreated control,but kept susceptible to chlorpyrifos and beta-cypermethrin.When the 3rd instar larvae were treated with chlorantraniliprole at concentration of LC10 and LC25 for 24,48 and 72 h,the specific activity of carboxylesterase（CarE） was induced,but the specific activity of cytochrome P450 O-deethylase（ECOD）,glutathione S-transferase（GSTs） and aryl amidase were significantly inhibited.After continuous treatment with LC10 and LC25 of chlorantraniliprole for 5 generations,the CarE and ECOD activity of the DBM were increased significantly to 1.16-,1.40-fold and 1.65-,1.56-fold respectively,whereas the specific activity of GSTs and aryl amidase was decreasedby 11.0%,27.5% and 43.6%,52.5% respecitively,comparing to those of the untreated control.These results suggested that the diamondback moth may have high risk of developing resistance to chlorantraniliprole,and the carboxylesterase and mixed function oxidase may be involved in the resistance of the DBM to chlorantraniliprole.

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