中文摘要：

p38MAPK作为MAPK信号通路中关键的一类，对于树突细胞的成熟及激活等都具有至关重要的调节作用．首先利用生物信息学的方法预测了p38可能受到一系列microRNA的调控，进一步利用双荧光素酶报告载体系统证实了miR-128可直接作用于p38基因的3’非编码区域并抑制p38的表达．转染miR-128可以很显著地降低树突细胞中p38的蛋白水平，进而引起细胞因子IL-6分泌的减少．因此，利用miR-128可能是改善树突细胞抗肿瘤免疫治疗的一个新策略．

英文摘要：

p38 MAPK, a subgroup of MAPK signaling pathway, plays a critical role in modulating den- dritic cell maturation and activation. Combined with bioinformatics analysis, p38 was predicted as potential tar- get of series of micro RNAs. Using dual luciferase reporter assays, demonstrated that miR-128 could repress p38 expression by binding to the 3＇UTR of p38 in a direct and sequence-specific manner. Moreover, transfection of miR-128 mimics could significantly decrease the protein level of p38 in dendritic cell, and reasonably decrease cytokines IL-6 production. Thus, uncovered the potential role of miR-128 to improve immunotherapies against tumors.