中文摘要：

【目的】对水稻lrd表达的光温诱导反应进行研究,明确水稻lrd表达光诱导波段和温度的抑制或促进作用。【方法】利用自有的6个水稻lrd突变体,用LED单色光源诱激和人工气候箱中不同温度梯度处理,观察类病斑的发生情况和统计病斑个数,比较诱导的效果。【结果】lrd31类病斑表达受紫外光诱导,lrd36的表达受黄光诱导,lrd37和lrd35的表达受蓝光诱导,而lrd32和lrd40在所有处理的光源下均表达类病斑;lrd32、lrd36和lrd37等3种温度下都正常产生病斑,低温明显促进病斑的发生。lrd35和lrd40在高温下不表达,在中温下可正常表达,低温下也表现促进作用,但lrd31只有在中温下正常表达,低温和高温抑制病斑出现。【结论】不同的lrd基因型所需的光诱导反应波段是不相同的。温度是抑制或促进lrd表达的另一个重要环境因子,一般是较低的温度促进lrd表达,高温抑制lrd表达。

英文摘要：

【Objective】 To determine the spectral range of light required for lesion formation and the effect of temperature on the expression of lrd lesions,the response of lrd expression to light and temperatures was tested for certain mutants in Oryza sativa L. 【Method】 Six lrd mutants derived from Indica and Japonica by gamma-ray mutagenesis simultaneously were illuminated by Light Emitting Diode （LED） light and were cultured in two different growth chambers set at three different temperature regimes. Lesions occurred on leave were observed and photographed for comparison the effect of different treatments. 【Result】 The expression of lrd lesions for lrd31 was induced by UV light,that of lrd36 was induced by yellow light,that of lrd37and lrd35 was induced by blue light,and lrd32 or lrd40 expressed lesion under all illuminated light. lrd32,lrd36 and lrd37 formed lesion under all culturing temperatures,and cooler environment accelerated their lesion expression,lrd35 and lrd40 were suppressed at high temperature and expressed at intermediate temperature,also accelerated under lower temperature. But lrd31 formed lesion only under intermediate temperature,and no lesion formation at high or lower culturing temperature. 【Conclusion】 The expressions of lrd lesions are light-dependent,different genotypes of lrd mutants in rice need specially spectral range of light. Temperature is another factor influencing lrd expression. The lower temperature seemed to enhance the expression of lrd phenotype.