中文摘要：

目的观察resistin基因过表达对3T3-L1脂肪细胞的脂质代谢、糖代谢的影响。方法构建大鼠resistin真核表达载体并转染3T3-L1前体脂肪细胞,获得稳定表达resistin基因的细胞株;采用油红O染色,观察脂肪细胞分化及脂质积聚情况;采用逆转录PCR技术,检测脂肪细胞分化标志基因及葡萄糖转运体4（glucose transporter4,GLUT4）基因表达变化;采用全自动生化仪比色法,检测脂肪细胞内甘油三酯（triglyceride,TG）、游离脂肪酸（free fatty acids,FFAs）的含量变化。结果（1）resistin基因过表达脂肪细胞中,脂滴出现时间提前,且细胞内布满了小而多的圆形脂滴;（2）resistin基因过表达脂肪细胞中,分化中、晚期标志基因C/EBPα、FAS的mRNA表达水平明显上调,分化早期标志基因Pref-1的表达则明显下调;（3）re-sistin基因过表达脂肪细胞中,胞质内TG、FFAs含量均显著增加;（4）resistin基因过表达脂肪细胞中,分化第2、4、8d的GLUT4基因mRNA表达水平间无显著变化,与正常脂肪细胞中的表达水平差异也无统计学意义。结论resistin基因过表达能够显著干扰3T3-L1脂肪细胞的脂质代谢,有助于肥胖和胰岛素抵抗的发生,而并不影响GLUT4基因的表达。

英文摘要：

Objective To investigate the effect of resistin overexpression on 3T3-L1 adipocyte lipid and glucose metabolism. Methods Expression vector for rat resistin gene was constructed and transfected into 3T3-L1 adipocytes. Cell differentiation and lipid accumulation was determined by Oil Red O staining. Differentiation marker genes （pref-1, C/EBPa, FAS ） and glucose transporter 4 （ GLUT4 ） gene mRNA expressions were evaluated by reverse transcription- PCR （RT-PCR）. Triglyceride （TG） and free fatty acids （FFAs） in adipocytes were measured by colorimetric kit. Results （1） In resistin-overexpressed adipocytes, the hpid droplets presented at the second day which was earlier than the control cells. （2） The expression of C/EBPα and FAS genes in resistin-overexpressed adipocytes were up-regulated and the pref-1 was down-regulated compared with that of the control cells. （3） In resistin-overexpressed adipocytes, cellular TG and FFAs levels were significantly increased （ P 〈 0.05）. （4） There was no difference in the expression of GLUT4 gene between 3T3-L1 adipocytes and resistin-overexpressed adipocytes （ P 〉 0.05）. Conclusion Overexpression of resistin can affect 3T3-L1 adipocyte hpid metabolism and thereby result in obesity and insulin resistance, but have no effect on GLUT4 gene expression.