中文摘要：

目的：观察隔药灸命门穴治疗对痛经模型大鼠子宫内膜的孕激素受体（PR）、雌激素受体（ER）表达量的作用机制。方法：将雌性SD大鼠随机分为空白组（A组）和造模组,构建原发性痛经的老鼠模型,造模成功后,将痛经老鼠模型随机分为模型组（B组）、药物组（C组）、针刺组（D组）、隔药灸组（E组）。观察各组大鼠出现扭体反应的潜伏期、30min内扭体次数及大鼠子宫内膜PR、ER表达水平。结果：模型组发生扭体反应次数较多,且扭体反应前有潜伏期,空白组无扭体反应（P〈0.01）。针刺组、药物组、隔药灸组大鼠扭体反应次数均较模型组减少,且扭体潜伏期均延长（P〈0.01）。隔药灸组和针刺组大鼠扭体反应次数显著降低（P〈0.01）;隔药灸组大鼠扭体反应较针刺组少（P〈0.01）。隔药灸组大鼠扭体抑制率最高（P〈0.05）。隔药灸组、针刺组、药物组及模型组大鼠ER表达较空白组显著增加,PR表达显著减少（P〈0.01）;隔药灸组、针刺组及药物组大鼠ER表达较模型组显著减少,PR表达显著增加（P〈0.01）;针刺组及隔药灸组大鼠ER表达较药物组显著降低,PR表达显著增加（P〈0.01）;隔药灸组大鼠ER表达较针刺组显著减少（P〈0.01）,PR表达显著增加（P〈0.01）。结论：隔药灸治疗原发性痛经与调节ER、PR表达有关,可下调痛经大鼠ER表达,提高PR表达。

英文摘要：

Objective： To observe the effect of medicinal-cake-seperated moxibustion on expression oi Progesterone receptor （PR）and Estrogen receptor （ER） in uterus of rat model with primary dysmenorrhea. Methods： Female rats were randomised into Model Group and Control Group（A Group）,and female model rats were estsblished. The blank control group,and female rats were divided into four group, model group （B Group） , medicine group（C Group）, acupuncture group （D Group）, drug-paste interposed moxibusion （E Group）. The latency period and the incidence rate of writhing body within 30 minutes were observed in each group and the serum levels of ER and PR were detected by Immuno histochemical method. Results： The lantency period of writhing body was shorter and the incidence rate of writhing body was more in model group, compared with those of A Group（P〈0.01）. The latency period of writ- hing body was longer and the incidence rate of writhing body was less in C group,D group and E group,compared with those of B group（P〈0.01）. But compared with C Group,the incidence rate of writhing body was less in D Group（P〈0.01）and it was least in E Group（P〈0.01）. Compared with C,D and E Group,the control rate of writhing body was highest in E Group Compared with A Group,the levels of ER in B.C.D and E Group were obviously ascend （P〈0.01） ,while the level of PR was obviously descend （P〈0.01） ;Compared with B Group,the levels of ER in C,D and E Group were obviously descend （P〈0.01） ,while the level of PR was obviously ascend （P〈0.01） ;Compared with D Group , the level of ER in E Group was obviously descend （P〈0.01）, while the level of PR was obviously ascend （P〈0.01）. Conclusion：The mechanisms of drug-paste interposed moxibustion treating with primary dysmenorrheal might be concerned with decreasing the level of ER,increasing the level of PR.