以高沙土和黄泥土为供试土壤,采用塑料盆分装土样,分别加入3个浓度的Cu^2+(100、200、300mgkg^-1)或Cd^2+(5、10、15mgkg^-1)模拟土壤污染,待这些污染土壤平衡培养2mo后,设置接种蚯蚓(Pheretima guiUemi Michaelsen)处理与不接种蚯蚓处理,并种植高丹草(Sorghum×Sudan,Sorghum bicolor× S. sudanense ) ;采用Tessler连续提取法测定重金属各结合形态,研究蚯蚓活动对土壤中Cu、Cd的主要形态及高丹草有效性的影响,以揭示蚯蚓对铜、镉污染土壤的修复作用.结果表明,蚯蚓活动显著提高了高沙土中碳酸盐结合态铜(Carb—Cu)和铁锰氧化物结合态铜(OxMncu)的含量;显著提高了Cd处理为10、15mg kg^-1的高沙土中离子交换态镉(Exch-Cd)和碳酸盐结合态镉(Carb—Cd)的含量,显著提高了黄泥土离子交换态铜(Exch—Cu)和有机结合态铜(WBO—Cu)的含量;通过测定各处理高丹草Cu、Cd含量发现,接种蚯蚓后,高沙土各Cu处理的高丹草的Cu含量显著增加,高沙土5、10mgkg^-1 Cd处理的高丹草的Cd含量以及黄泥土各Cd处理的高丹草的Cd含量均显著增加.图3表3参32
The objective of this study was to investigate the effect of earthworm activities on Cu and Cd forms in contaminated soils (sandy soil from the middle of Jiangsu Province and permeable paddy soil from the south of Jiangsu Province) and their availability to Sorghum bicolor × S. sudanense. Each soil was amended and contained by Cu^2 + 0, 100,200 and 400 mg kg^ - 1 and Cd^2+ 0, 5, 10 and 20 mg kg^- 1 by adding appropriate concentrations of CuSO4 and CdSO4, respectively. After the metal - treated soil was incubated for two months, fully matured earthworms (Pheretima guillemi Michaelsen) were raised in the soil of half pots, while the other half pots contained only soil, but no earthworms. Pre-germinated grass seeds (Sorghum bicolor × S. sudanense ) were subsequently sown in the pots with and without earthworms. The study indicated that the activities of earthworms significantly increased the contents of Carb-Cu, OxMn-Cu and Exch-Cd, and Car-Cd in sandy soil. However, the contents of Exch-Cu and WBO-Cu were increased in permeable paddy soil. Results also indicated that the earthworms could apparently affect Cu accumulation in Sorghum bicolor × S. sudanense planted only in sandy soil, but increased the content of Cd both in sandy soil and permeable paddy soil. Fig 3, Tab 3, Ref 32