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Isolation and functional analysis of transcription factor GmWRKY57B from soybean
  • ISSN号:1672-1810
  • 期刊名称:《植物遗传资源学报》
  • 时间:0
  • 分类:S52[农业科学—作物学]
  • 作者机构:[1]Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China, [2]Department of Biology, Harbin Normal University, Harbin 150025, China
  • 相关基金:Supported by the National Basic Research and Development Program of China (Grant No. 2004CB117200) and the Basic Research Programme of Education of Heilongjiang Province (Grant No. 10541088)
中文摘要:

WRKY 蛋白质是在涉及许多生物过程的更高的植物的 transcriptional 管理者的一个大家庭,例如对关於生命、不能生活的应力,植物发展和新陈代谢的回答。在这研究,一个大豆 cDNA 图书馆被构造并且由酵母一个混血儿屏蔽了系统使用 3 × W 框元素作为一个诱饵,并且因而一个抄写因素,命名 GmWRKY57B,组 3 WRKY 家庭的一个成员,被获得。GmWRKY57B 明确地在酵母房间绑在 Arabidopsis NPR1 (AtNPR1 ) 倡导者的 W 盒子。这特定的绑定进一步被证实与一在里面 vitro electrophoretic 移动试金。RT-PCR 分析证明 GmWRKY57B 大豆工厂在根在类似的水平被表示,起源并且长叶。在烟草的 GmWRKY57B 基因的 Overexpression 授与 transgenics 公差到干旱应力。这结果显示 GmWRKY57B 基因在工厂忍耐起一个作用到不能生活的应力。

英文摘要:

WRKY proteins are a large family of transcriptional regulators in higher plants involved in many biological processes, such as responses to biotic and abiotic stress, plant development and metabolism. In this study, a soybean cDNA library was constructed and screened by yeast one-hybrid system using the 3xW-box element as a bait, and consequently a transcription factor, named GmWRKY57B, a member of group 3 of WRKY family, was obtained. GmWRKY57B specifically binds to the W-box of the Arabidopsis NPR1 (AtNPR1) promoter in yeast cells. This specific binding was further confirmed with an in vitro electrophoretic mobility shift assay. RT-PCR analysis showed that GmWRKY57B was expressed at a similar level in root, stem and leaf of the soybean plant. Overexpression of the GmWRKY57B gene in tobacco conferred the transgenics tolerance to drought stress. This result indicated that the GmWRKY57B gene plays a role in plant tolerance to abiotic stress.

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期刊信息
  • 《植物遗传资源学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院作物科学研究所 中国农学会
  • 主编:刘旭
  • 地址:北京中关村南大街12号
  • 邮编:100081
  • 邮箱:zwyczyxb2003@163.com/sina.com/caas.cn
  • 电话:010-82105794 82105795
  • 国际标准刊号:ISSN:1672-1810
  • 国内统一刊号:ISSN:11-4996/S
  • 邮发代号:82-643
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:10278