WRKY 蛋白质是在涉及许多生物过程的更高的植物的 transcriptional 管理者的一个大家庭,例如对关於生命、不能生活的应力,植物发展和新陈代谢的回答。在这研究,一个大豆 cDNA 图书馆被构造并且由酵母一个混血儿屏蔽了系统使用 3 × W 框元素作为一个诱饵,并且因而一个抄写因素,命名 GmWRKY57B,组 3 WRKY 家庭的一个成员,被获得。GmWRKY57B 明确地在酵母房间绑在 Arabidopsis NPR1 (AtNPR1 ) 倡导者的 W 盒子。这特定的绑定进一步被证实与一在里面 vitro electrophoretic 移动试金。RT-PCR 分析证明 GmWRKY57B 大豆工厂在根在类似的水平被表示,起源并且长叶。在烟草的 GmWRKY57B 基因的 Overexpression 授与 transgenics 公差到干旱应力。这结果显示 GmWRKY57B 基因在工厂忍耐起一个作用到不能生活的应力。
WRKY proteins are a large family of transcriptional regulators in higher plants involved in many biological processes, such as responses to biotic and abiotic stress, plant development and metabolism. In this study, a soybean cDNA library was constructed and screened by yeast one-hybrid system using the 3xW-box element as a bait, and consequently a transcription factor, named GmWRKY57B, a member of group 3 of WRKY family, was obtained. GmWRKY57B specifically binds to the W-box of the Arabidopsis NPR1 (AtNPR1) promoter in yeast cells. This specific binding was further confirmed with an in vitro electrophoretic mobility shift assay. RT-PCR analysis showed that GmWRKY57B was expressed at a similar level in root, stem and leaf of the soybean plant. Overexpression of the GmWRKY57B gene in tobacco conferred the transgenics tolerance to drought stress. This result indicated that the GmWRKY57B gene plays a role in plant tolerance to abiotic stress.