中文摘要：

目的探讨GSTM1和GSTT1基因多态性对苯乙烯接触工人苯乙烯代谢的影响。方法于2006年11月选择山东省某玻璃钢游艇生产企业喷漆车间工龄在1年以上、防护情况基本相同的全部工人共计56人作为研究对象。分别测定个体苯乙烯接触剂量、尿中代谢产物——苯乙烯巯基尿酸的浓度，采用多重PCR技术检测GSTM1和GSTT1基因多态性。结果工人尿中苯乙烯巯基尿酸浓度均值为（19．80±13．25）mg／gCr，苯乙烯8h时间加权平均浓度f8h-TWA）均值为（133．28±95．81）mg／m3，两者间呈正相关（r=0．937，P〈0．05）。高暴露组（苯乙烯浓度〉50mg／m3）和低暴露组f苯乙烯浓度≤50mg／m3）工人年龄、工龄、吸烟率、饮酒率以及GSTM1、GSTT1基因型的构成比间比较，差异均无统计学意义。无论在低暴露组还是高暴露组，携带GSTMI（＋）基因型受试工人尿中的苯乙烯巯基尿酸浓度均高于携带GSTMI（-）基因型受试工人，差异均有统计学意义（P〈0．05）；但是在携带GSTTl不同基因型受试工人之间中未发现同等差异。结论CSTM1是苯乙烯的主要代谢酶之一，其基因多态性可影响苯乙烯在体内的代谢过程。

英文摘要：

Objective To explore the influence of genetic polymorphisms of GSTM1 and GSTT1 on metabolism of styrene in styrene-exposed workers. Methods In November 2006,fifty-six styrene-exposed workers were chosen as the study subjects. Personal passive samplers were used to determine personal 8-hour-time-weighted average （8h-TWA）. The concentration of the phenyl-hydroxyethyl-mercapturic-acid （PHEMA） in urine was determined by Ellman spectrophotometry. The genetic polymorphisms of GSTT1 and GSTM1 were detected by multiple PCR method. Results The mean concentration of PHEMA in urine,8-hour-TWA of styrene were （19.80~13.25） mg/g Cr,（133.28~95.81） mg/m3,respectively, with a positive correlation （r=0.937,P〈0.05）. No significant correlation was observed in age, working age, the rates of smoking and drinking, the proportions of GSTM1 genotypes,the proportions of GSTT1 genotypes in between high and low exposed groups. Whether in high-exposed group （8h-TWA〉50 mg/m3） or low-exposed group （8h-TWA≤50 mg/m3）,there was a significant association between the concentration of the PHEMA in urine and genotypes of GSTM1 （P〈0.05）. Conclusion GSTM1 is one of the key enzymes in metabolism of styrene in human body. Genetic polymorphisms of GSTM1 can influence metabolic process of styrene.