中文摘要：

神经肽Y（Neuropeptide Y,NPY）在机体的摄食活动中发挥重要作用,是哺乳动物最重要的一种内源性促食欲因子。为了解大口黑鲈（Micropterus salmoide）NPY基因的结构及进一步研究该基因在大口黑鲈中的功能作用,采用RT-PCR和RACE技术,克隆了大口黑鲈北方亚种（M.salmoides salmoides）和佛罗里达亚种（M.salmoides floridanus）NPY基因c DNA序列,结果表明两亚种NPY c DNA均包括一个编码99个氨基酸的ORF框和长度为52 bp的5＇非编码区（5＇-UTR）;采用PCR和基因组步移技术获得了长度分别为3 561 bp和3 565 bp的大口黑鲈北方亚种和佛罗里达亚种NPY基因DNA序列。序列分析结果表明,大口黑鲈北方亚种和佛罗里达亚种NPY基因由4个外显子和3个内含子组成。经MATINSPECTOR软件预测,在北方亚种和佛罗里达亚种启动子序列分布有TATA框、CAAT框、CCAAT-Box、GATA-Box等基本转录调控元件。实验在大口黑鲈两亚种NPY DNA序列间发现了6个单个位点碱基差异,与大口黑鲈北方亚种相比佛罗里达亚种启动子区域出现一个4个碱基的插入。不同物种间NPY基因的序列同源性分析表明大口黑鲈与鳜鱼和石斑鱼的NPY基因核苷酸同源性最高,达90%和88%,氨基酸同源性分别为93%和95%。

英文摘要：

Neuropeptide Y（NPY）plays an important role in the activity of body feeding,and is the most potent endogenic orexigenic factor in mammals. In order to understand the structure and the function of NPY gene in the largemouth bass（Micropterus salmoide）,the NPY＇s c DNA of northern and Florida subspecies of largemouth bass were cloned by RT-PCR and RACE techniques. The result revealed that the NPY＇s c DNA in two subspecies all contained an ORF encoding 99 amino acids and 5 ′-UTR of 52 bp. Using PCR and genomewalker technology,a 3 561 bp and a 3 565 bp DNA sequence of of NPY gene were acquired from northern and Florida subspecies of largemouth bass respectively. Sequence analysis indicated that gene from both subspecies contained 4 exons and 3 introns. By bioinformatics analysis with MATINSPECTOR,the basic transcriptional regulatory elements such as TATA Box,CAAT Box,CCAAT Box,and GATA Box were found in the promoters of both subspecies. Six single-site base differences between the two genomic NPY DNA sequences of 2 subspecies were discovered,4-base insert segments were identified in promoter sequence of Florida subspecies compared with the northern one. The homology of NPY genes with other species was also compared,the largemouth bass shared the highest nu cleotide homology with mandarin fish and grouper,reached 90% and 88% respectively,and the amino acid homology were 93% and 95%.